REVIEW PAPER
In vitro differentiation of preadipocytes from bovine
adipose tissue
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1
Facultad de Estudios Superiores – Cuautitlán, UNAM, México
2
Centro de Investigación y Estudios Avanzados - Zacatenco, México
3
Instituto de Neurobiología–UNAM, México
Publication date: 2004-08-30
Corresponding author
O. Mora
Facultad de Estudios Superiores – Cuautitlán, UNAM, México
J. Anim. Feed Sci. 2004;13(Suppl. 1):389-392
KEYWORDS
ABSTRACT
To study differentiation of bovine adipose tissue preadipocytes into adipocytes, two tissue sources,
two animal ages, and two differentiation media were tested. While no differentiation was observed with
cells obtained from adult adipose tissue, foetal perirenal fibroblasts differentiated into adipocytes in
the following medium: DMEM/F-12 1:1; 10 µg/mL insulin; 0.25 µM dexamethasone; 10 µg/mL very
low-density lipoproteins; 100 µg/mL streptomycin; and 100 U/mL penicillin.