In the present study, Caecomyces sp., an anaerobic rumen fungus from faeces of the Indian elephant, was immobilized using calcium-alginate. The resulting beads were found to solubilize within 4-5 h in culture medium at 39°C (pH 6.9), indicating their possible dissociation in the rumen, if used as a feed additive. Since media was also used as a component of the immobilization material, the fungi were not only found to survive, but also to grow, as mycelial growth was visible macroscopically on the surface of the beads. Incubation of immobilized fungi with wheat straw at different temperatures was found to extend the viability of immobilized fungi. Immobilization of rumen fungi and their subsequent incubation with an appropriate substrate (e.g., wheat straw, etc.) could be used to enhanced their survival, industrial use as propagules, and as direct-fed microbials/ feed additives for domesticated ruminants.