ORIGINAL PAPER
A highly efficient method for derivatization of fatty
acids for high performance liquid chromatography
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The Kielanowski Institute of Animal Physiology and Nutrition,
Polish Academy of Sciences,
05-110 Jabłonna, Poland
Publication date: 2002-08-02
J. Anim. Feed Sci. 2002;11(3):517-526
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ABSTRACT
A new derivatization procedure for fatty acids followed by HPLC analysis with UV detection is
described. Originally, derivatization was carried out with acetone solutions of 2,4'-dibromoacetophenone and triethylamine at 50°C for 2h. To prevent oxidation and isomerization, unsaturated fatty acids and conjugated dienes in particular were derivatized at a lower temperature (40°C) for 30
min using a concentrated solution of 2,4'-dibromoacetophenone in acetone and triethylamine. The
new derivatization method decreases the risk of degradation and isomerization of unsaturated fatty acids. Due to the use of higher concentrations of substrates in reaction mixtures, significantly
enhanced sensitivity of fatty acid analysis is observed. In contrast to the original derivatization
method, the new one can be successfully used for both large and trace concentrations of fatty acids
in various types of biological materials.
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