SHORT COMMUNICATION
A highly efficient method for determination of
allantoin in ruminal digesta, ovine urine and
blood serum by HPLC
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The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences,
05-110 Jabłonna, Poland
Publication date: 2004-09-16
Corresponding author
M. Czauderna
The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences,
05-110 Jabłonna, Poland
J. Anim. Feed Sci. 2004;13(Suppl. 2):67-70
KEYWORDS
ABSTRACT
A sensitive HPLC method for direct determination of allantoin in ruminal digesta, urine and
blood serum of sheep is described. Blood serum and urine samples were diluted 1:3 with deionized
water. Separation and quantification of allantoin was achieved using three long Nova Pac C18-columns (Waters) and UV detection at 205 and 215 nm. The Iow coefficient of variation and satisfactory recovery of allantoin added to assayed samples (~100%) provide a suitable tool for routine
quantification of allantoin in a large number of urine, ruminal digesta and blood serum samples.
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