A highly efficient method for determination of
allantoin in ruminal digesta, ovine urine and
blood serum by HPLC

A sensitive HPLC method for direct determination of allantoin in ruminal digesta, urine and blood serum of sheep is described. Blood serum and urine samples were diluted 1:3 with deionized water. Separation and quantification of allantoin was achieved using three long Nova Pac C₁₈-columns (Waters) and UV detection at 205 and 215 nm. The Iow coefficient of variation and satisfactory recovery of allantoin added to assayed samples (~100%) provide a suitable tool for routine quantification of allantoin in a large number of urine, ruminal digesta and blood serum samples.

CITATIONS (1):

Simultaneous determination of chondroitin sulfate sodium, allantoin and pyridoxine hydrochloride in pharmaceutical eye drops by an ion-pair high-performance liquid chromatography
Pengfei Jin, Jie Ma, Xuejun Wu, Ding Zou, Chunhua Sun, Xin Hu
Journal of Pharmaceutical and Biomedical Analysis

CrossRef

Submit your paper

Instructions for Authors

Archive

Send by email

Regulation of Nod-like receptor expression in the liver of ewes during early pregnancy

The role of sheep’s milk bioactive substances in the prevention of metabolic and viral diseases

Changes in nuclear factor kappa B components expression in the ovine spleen during early pregnancy

Brain-derived neurotrophic factor (BDNF) affects somatotrophic axis activity in sheep

Changes in the content and intensity of oxidation of lipid compounds in the kidneys of lambs fed diets with rapeseed and fish oils – effect of antioxidant supplementation

Indexes

Keywords index

Topics index

Authors index

ISSN:

1230-1388

Assigning DOI numbers, introducing articles from supplements and recent publications to POL-index database, maintaining anti-plagiarism detection, electronic system to proceed manuscripts and webpage of the Journal with interactive pdf files, and language correction of manuscripts published in Journal of Animal and Feed Sciences are financed in the years 2018–2019 by the Ministry of Science and Higher Education from the funds for science popularization activities, Agreement No. 631/P-DUN/2018.