Activity and stability of a fungal
1,4-beta-endo-xylanase preparation
in the rumen of sheep

Szent István University, Faculty of Veterinary Science, Department of Animal Hygiene, Herd Health and Veterinary Ethology, István u 2., H-1078 Budapest, Hungary

Research Institute for Animal Breeding and Nutrition, Gesztenyés u 1., H-2053 Herceghalom, Hungary

Publication date: 2002-10-11

Corresponding author

P. Rafai

Szent István University, Faculty of Veterinary Science, Department of Animal Hygiene, Herd Health and Veterinary Ethology, István u 2., H-1078 Budapest, Hungary

J. Anim. Feed Sci. 2002;11(4):627-635

DOI: https://doi.org/10.22358/jafs/67918/2002

Article (PDF)

Citations (2)

KEYWORDS

xylanase

Thermomyces lanuginosus

Remazol Blue

ABSTRACT

Experiments were conducted with 1-3 years old, rumen cannulated Merino wethers to characterize the stability of the 1,4-β-endo-xylanase activity of a commercially available enzyme preparation (Rumino-Zyme, 250 FXU/g xylanase activity, Dr. Bata Ltd., Hungary). The Remazol blue technique used for determination of the xylanase activity of the rumen ﬂuid and solid samples proved simple, quick and reliable. The xylanase activity in the rumen of fasting wethers was found to be 20-30 FXU/l. It was by about 2.5 times greater two and four hours after feeding than the value measured pre-feeding. Exogenous xylanase added directly into the rumen of fasted wethers caused an increase in the enzyme activity. After the peak activity at 5 min after treatment the enzymatic activity decreased with an estimated half-life of 45-50 min. This result of the present investigation did not support the earlier observations, which indicated lasting activity of external NSP-enzymes in vivo.

CITATIONS (2):

Rumen fermentation response to a direct-fed xylanase enzyme preparation fromThermomyces lanuginosusin sheep
V. Jurkovich, J. Kutasi, Hedvig Fébel, J. Reiczigel, E. Brydl, L. Könyves, P. Rafai
Acta Veterinaria Hungarica

CrossRef

Increasing doses of carbohydrases: Effects on rumen fermentation, nutrient digestibility, and performance of mid-lactation cows
Paulo Vittorazzi, Julia Marques, Caio Takiya, Rodrigo Chesini, Milena Bugoni, Silva da, Alanne Nunes, Tássia Silva, Mauro Dias, Nathalia Grigoletto, Cristina Cortinhas, Tiago Acedo, Francisco Renno
Journal of Dairy Science

CrossRef

Submit your paper

Instructions for Authors

Archive

Send by email

Influence of fibrolytic enzymes mixture on performance, nutrient digestion, rumen fermentation and microbiota in Holstein bulls

Changes in fibrolytic microbes and enzymes of fistulated goat in response to feeding with methanol extract of Sapindus rarak

Effect of exogenous fibrolytic enzymes on in vitro rumen fermentation of tropical forages

Nutritional impact on intestinal bacterial communities of pigs studied by molecular biology techniques

Gastrointestinal responses of turkeys to the addition of exogenous xylanase and glucanase to diets

Indexes

Keywords index

Topics index

Authors index

ISSN:

1230-1388

Assigning DOI numbers, introducing articles from supplements and recent publications to POL-index database, maintaining anti-plagiarism detection, electronic system to proceed manuscripts and webpage of the Journal with interactive pdf files, and language correction of manuscripts published in Journal of Animal and Feed Sciences are financed in the years 2018–2019 by the Ministry of Science and Higher Education from the funds for science popularization activities, Agreement No. 631/P-DUN/2018.