Assessment of the ability of dietary yeast-fermented rapeseed
meal to modulate inflammatory and oxidative stress
in piglets after weaning

The aim of the study was to investigate the potential of a diet containing rapeseed meal fermented by Saccharomyces cerevisiae as a new sustainable feed to reduce transient intestinal inflammation, diarrhoea and oxidative stress in piglets after weaning. In this study, 16 male post-weaning piglets, with an initial weight of 9.04 ± 0.19 kg, were randomly allocated to two dietary treatments: control and 10% fermented rapeseed meal (FRSM) – 8 pigs/treatment. The experiment lasted 21 days. At the end of the trial, the animals were slaughtered and samples of blood and segments of the jejunum, ileum and colon were collected for determination of plasma biochemical, inflammatory and oxidative stress parameters. Pig performance and diarrhoea incidence were also investigated. The results showed that the FRSM diet had no significant effect on piglet performance, weight and average daily weight gain, as well as plasma biochemical parameters. However, the number of piglets with diarrhoea was higher in the control group than in the group receiving the FRSM diet throughout the experimental period. Moreover, a decrease in TNF-α (P = 0.03) and IL-1ß (P < 0.05) cytokine levels was recorded in the colon and jejunum samples from the FRSM group. In addition, IL-8 and IL-6 concentrations were decreased (P = 0.0009 and P = 0.03, respectively) in the ileum of piglets fed FRSM, indicating the modulatory capacity of this feed in reducing weaning-associated intestinal inflammation. The FRSM diet also improved the antioxidant status and significantly reduced lipid peroxidation and thiobarbituric acid reactive substance levels in plasma (P = 0.022) and in the jejunum (P = 0.028) and colon (P = 0.003), suggesting the potential of fermented rapeseed meal to limit oxidative reactions. In conclusion, the present study showed that fermentation of rapeseed meal using S. cerevisiae enriched the nutrient composition and reduced the concentration of anti-nutrients (e.g. glucosinolates). Moreover, the addition of FRSM to diets of pigs after weaning improved their intestinal health status, indicating its beneficial effect.

FUNDING

This research was funded by the Romanian Ministry of Education and Research under the project No. PN-III-P2-2.1-PED/396-2020.

CONFLICT OF INTEREST

The Authors declare that there is no conflict of interest.

METADATA IN OTHER LANGUAGES:

Chinese

酵母发酵菜籽粕对断奶仔猪炎症和氧化应激调控能力的评价

摘要：本研究旨在探讨含酵母菌发酵的菜籽粕作为一种新型可持续饲料降低仔猪断奶后短暂肠道炎症、腹泻和氧化应激的潜力。试验选取16头初始体重为9.04 ± 0.19 kg的断奶后公仔猪，随机分为对照组和10%发酵菜籽粕（FRSM）组（8头/组），试验期为21 d。试验结束后，屠宰动物，采集血液及空肠、回肠和结肠部分样本，测定血浆生化、炎症和氧化应激参数。并对猪的生产性能和腹泻发生率进行了调查。结果表明：FRSM饲喂对仔猪生产性能、体重、平均日增重及血浆生化指标均无显著影响。试验期，对照组仔猪腹泻数均高于FRSM组。此外，FRSM组结肠和空肠标本中TNF-α（P = 0.03）和IL-1ß（P < 0.05）细胞因子水平下降。FRSM降低了仔猪回肠IL-8和IL-6浓度（P = 0.0009和P = 0.03），表明FRSM对降低断奶相关性肠道炎症具有调节作用。FRSM饲喂还改善了抗氧化状态，显著降低了血浆（P = 0.022）、空肠（P = 0.028）和结肠（P = 0.003）脂质过氧化和硫代巴比妥酸反应物质（TBARS）水平，表明发酵菜籽粕具有抑制氧化反应的潜力。综上所述，FRSM可丰富其营养成分，降低抗营养物质（如硫代葡萄糖苷）的浓度。此外，仔猪断奶后饲粮中添加FRSM改善了肠道健康状况，表明其对肠道具有有益的作用。

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