Seeds of six cultivars of yellow lupin (Lupinus luteus L.), three of white lupin (Lupinus albus L.) and four of narrow-leaved lupin (Lupinus angustifolius L.) were analysed for the content of nutrients and antinutritional factors. Batches of seeds harvested during three consecutive years were analysed for each cultivar. Seeds of yellow lupin cultivars contained significantly (P<0.05) more protein (43.5% DM), cystine (2.21 g/16gN), ash (5.4% DM), crude fibre (16.4% DM) and alkaloids (510-1300 mg/kg DM) than seeds of white and narrow-leaved lupins. Seeds of white lupin had significantly more fat (10.5% DM), manganese (670 mg/kg DM), toxic alkaloids (600-950 mg/kg DM) and more threonine and isoleucine (3.77 and 4.39 g/16g N , respectively) as compared to other species. The contents of lysine, cystine and isoleucine in yellow lupin protein and that of threonine in white and narrow-leaved lupin seeds (g/16g N) were negatively correlated with the protein content of seeds.