Detection and  in vitro  regulation of NHE1 and
NHE3 mRNA expression in rumen epithelia of
cows

NHE (Na⁺/H⁺ exchanger) mediates electroneutral Na transport across rumen epithelium by ejection of intracellular H⁺ in exchange of external Na⁺ with a ratio of 1:1. It contributes to the absorption of Na⁺ and maintenance of intracellular pH homeostasis of rumen epithelium. This study explored the existing of NHEs in rumen epithelia of cows and the responses of NHE1 and NHE3 mRNA to IGF-1. The results, obtained by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR), illustrated that NHE1 and NHE3 mRNA is present in rumen epithelia of cows. The NHE1 and NHE3 mRNA expression were promoted by IGF-1 treatment (25 ng/ml) in vitro. The abundance of NHE1 and NHE3 mRNA were significantly higher in IGF-1 treated groups (P<0.05). Taking together, the current study revealed that the mRNA of NHE1 and NHE3 in rumen epithelia of cows could be positively regulated by IGF-1.

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1230-1388

Assigning DOI numbers, introducing articles from supplements and recent publications to POL-index database, maintaining anti-plagiarism detection, electronic system to proceed manuscripts and webpage of the Journal with interactive pdf files, and language correction of manuscripts published in Journal of Animal and Feed Sciences are financed in the years 2018–2019 by the Ministry of Science and Higher Education from the funds for science popularization activities, Agreement No. 631/P-DUN/2018.