SHORT COMMUNICATION
Determination of phytoestrogens in bovine
plasma using liquid chromatography and tandem
mass spectrometry
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Department of Animal Nutrition and Physiology,
Danish Institute of Agricultural Sciences, Research Centre Foulum,
P.O. Box 50, DK-8830 Tjele
Publication date: 2004-08-30
Corresponding author
J. Hansen-Møller
Department of Animal Nutrition and Physiology,
Danish Institute of Agricultural Sciences, Research Centre Foulum,
P.O. Box 50, DK-8830 Tjele
J. Anim. Feed Sci. 2004;13(Suppl. 1):315-318
KEYWORDS
ABSTRACT
Phytoestrogens have received an increasing interest due to their potential protective effect
against a number of human diseases. A method based on liquid-chromatography and negative
electrospray tandem mass spectrometry was developed for assaying phytoestrogens in blood plasma.
The detection limit was 0.1 to 3.1 nM for various isoflavones, lignans and mycotoxines. In a dairy
cow fed whiteclover grass the arterial plasma concentrations of the conjugated forms of enterodiol,
enterolactone, daidzein, and equol were 2, 440, 93 and 115 nM, respectively. The concentrations of
the non-conjugated forms were 0.4, 16, 0.8 and 1.1 nM, respectively. Formononetin and genistein
were detected only in the conjugated forms (1.6 and 22 nM). There were positive net portal fluxes of
all phytoestrogens detected. The liver extracted equol, but released daidzein in the non-conjugated
form. These preliminary data indicate important differences in liver metabolism of various
phytoestrogens in dairy cows.
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