Determining utilizable true protein digestibility of
mixed rations for sheep using  in vitro  techniques

The objective of the experiment was to compare in vitro digestion techniques, i.e. 24, 48 and 72 h pepsin digestion, pepsin-small intestinal fluid (SIF) digestion and pepsin-pancreatin digestion, for the determination of in vitro digestibility of utilizable true protein (uTP) of mixed rations for sheep. Twelve typical mixed rations for sheep (CP content 7.64-13.02% DM) were formulated as feed samples. It was found that there was no significant deference in uTP digestibility of mixed rations between 24, 48 and 72 h pepsin digestion (P>0.05), whereas uTP digestibility of pepsin-SIF treatment was higher than that of pepsin treatment (P<0.05), and uTP digestibility of pepsin-pancreatin treatment was higher than that of all other treatments (P<0.05). Further research is needed to study the reasons for the difference in in vitro uTP digestibility between pepsin digestion, pepsin-SIF digestion and pepsin-pancreatin digestion, and study the relationship between in vitro and in vivo uTP digestibility before a suitable in vitro technique for determining uTP digestibility is proposed.

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1230-1388

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