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ORIGINAL PAPER
Development of an enzyme-linked immunosorbent
assay for the determination of hexoestrol
1
School of Food Science and Technology, Southern Yangtze University,
214036, WuXi, JiangSu Province, P.R. China
2
Chinese Academy of Inspection and Quarantine,
100082, Beijing, P.R. China
Publication date: 2006-01-06
Corresponding author
Ch. L. Xu
School of Food Science and Technology, Southern Yangtze University, 214036, WuXi, JiangSu Province, P.R. China
School of Food Science and Technology, Southern Yangtze University, 214036, WuXi, JiangSu Province, P.R. China
J. Anim. Feed Sci. 2006;15(1):159-171
KEYWORDS
ABSTRACT
A competitive enzyme-linked immunoassay (ELSIA) was developed for the quantitative detection
of the hexoestrol (HES). Polyclonal rabbit antisera, raised against protein conjugate hexoestrol-mono-caroxyl-propyl-ethyl-bovine-serum-albumin (HES-MCPE-BSA), were utilized in immobilized
antibody-based and competitive immunoassays. Assay conditions, including concentrations of
antisera and horseradish peroxidase (HRP)-HES were optimized. The effect of incubation time,
surfactant concentration, ionic strength and pH of the medium were also investigated. The typical
calibration curve gave an average IC50 value of 2.4 ng/mL, calibration range from 0.2 ng/mL to 30.5
ng/mL and a detection limit of 0.07 ng/mL. The specificity of the assay was tested against HES
structurally related compounds, and the assay proved highly selective for HES. Assay performance
was validated by using spiked urine samples.
| ISSN: | 1230-1388 |
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