Development of nucleic acid based techniques and possibilities of their application to rumen microbial ecology research

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Policies General information Open Access, Licensing terms, Commercial use and Copyright terms policies Self-archiving policy and Archive policies Article Correction and Withdrawal policy Manuscript Submission policy Authorship policy Conflict of Interest policy Language considerations policy Plagiarism and Duplicate publications policy Ethics policy Review process policy Acceptance of manuscripts policy Online First Articles and Special Issues policies Generative artificial intelligence (AI) policy Advertising policy

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Development of nucleic acid based techniques and possibilities of their application to rumen microbial ecology research

E. Pers-Kamczyc ¹

,

P. Zmora ¹

,

A. Cieślak ¹

,

M. Szumacher-Strabel ¹

1

Poznan University of Life Sciences, Department of Animal Nutrition and Feed Management, RUMEN PULS, Wołyńska 33, 60-637 Poznań, Poland

Publication date: 2011-08-04

Corresponding author

E. Pers-Kamczyc

Poznan University of Life Sciences, Department of Animal Nutrition and Feed Management, RUMEN PULS, Wołyńska 33, 60-637 Poznań, Poland

J. Anim. Feed Sci. 2011;20(3):315-337

DOI: https://doi.org/10.22358/jafs/66189/2016

KEYWORDS

molecular techniques

ABSTRACT

Despite many years of research elucidating processes occurring in the rumen, still only around 10% of the rumen microbe is known. Molecular biology techniques seem to be a good complement of an information obtained by using the classical microbiology and therefore can enhance our knowledge about processes occurring in the rumen. The aim of this paper is to describe and to summarize a widely used techniques of the molecular biology during analysis of microbes in rumen. The main attention have been focused on the three of them which can be employed for quantitative and qualitative analysis of rumen microorganisms: PCR-DGGE, Real-time PCR and Fluorescence in situ hybridization.

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20.

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Annals of Animal Science

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