In this study, the effects of coenzyme Q₁₀ (CoQ₁₀) on growth performance, antioxidant status and organ weights in cold-stressed Japanese quails (Coturnix coturnix japonica) were investigated. During the experiment, a 2 × 3 factorial design was employed with two environmental temperatures (ET) and three levels of CoQ₁₀ (0, 20 and 40 mg/kg). A total of 180 one-day-old male quails were randomly allocated into 6 groups with 6 replicates with 5 birds in each replicate. The birds were fed in two separate rooms at either 22 ± 2 °C for 24 h/day (thermoneutral, TN) or 12 ± 2 °C for 8 h/day (cold stress, CS; between 09:00–17:00) followed by 22 ± 2 °C for 16 h/day. CoQ₁₀ addition into diet increased final body weight, body weight gain and cumulative feed intake only in CS regardless of the used dose. It was stated that CoQ₁₀ supplementation did not exert influence on serum and liver superoxide dismutase (SOD) activity and liver total antioxidant status (TAS) in TN conditions, but increased these parameters in CS; however in liver the higher CoQ₁₀ dose was required to obtain the statistically positive effect. When quails were exposed to CS a higher dose of CoQ₁₀ caused a more pronounced decreased in serum malondialdehyde (MDA) level than the lower one; however the effect of CoQ₁₀ on liver MDA level was shown regardless of the used dose. The obtained results show that CoQ₁₀ supplementation reverses the negative effects of CS on growth performance, antioxidant status and organ weights in quails. The caused may effects partly associated with direct antioxidant properties of CoQ₁₀ as well as the synergistic efficacy of CoQ₁₀ with SOD activity.