SHORT COMMUNICATION
Difference of nitrogen contents determined by the
combustion and Kjeldahl method in response to
nitrate nitrogen in some feedstuffs
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State Key Laboratory of Animal Nutrition, College of Animal Science & Technology,
China Agricultural University,
Beijing 100094, P.R. China
Publication date: 2007-09-17
Corresponding author
Q. X. Meng
State Key Laboratory of Animal Nutrition, College of Animal Science & Technology,
China Agricultural University,
Beijing 100094, P.R.. China
J. Anim. Feed Sci. 2007;16(Suppl. 2):178-183
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ABSTRACT
T
Two experiments were conducted to examine the difference of nitrogen contents determined by the
combustion (Cm) and Kjeldahl (Km) method and its response to nitrate nitrogen in ruminant feedstuffs. In
Experiment 1, 14 ruminant feedstuffs were determined for combustion nitrogen (Cn), Kjeldahl nitrogen
(Kn) and nitrate nitrogen (NO3-N). Although NO3-N resulted in a difference between the Cm and Km, the
correlation coefficient of the NO3-N content and the value of “Cn-Kn” was low (R2= 0.6341), suggesting
other factors influencing the difference of N determination between the Cm and Km. In Experiment 2,
recoveries of net NO3-N were determined using Chinese wild rye-grass (CWG), maize grain (MG) and
soyabean meal (SBM) supplemented with sodium nitrate at the level of 5, 10, 15, 20, 25, 30, 35, 40 and
45% on DM basis, respectively. The results showed that different recoveries of NO3-N by Km rather than
Cm would account for the difference of Cn and Kn in some ruminant feedstuffs.
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