From weaning to approximately 60 kg liveweight, 24 castrated pigs were assigned to one of 4 diets. The diets were barley-wheat-soyabean meal based and contained either 6% animal fat (diet 1, control), 3% animal fat + 3% fish oil (diet 2), 6% fish oil (diet 3), or 6% of a mixture of fish oil and coconut oil (2/1) (diet 4). The fish oil was an unrefined sand launce oil. Thereafter, all pigs were switched to the finisher-diet added 2% tallow, which was fed until slaughter at approximately 100 kg liveweight. Subcutaneous fat and muscle samples of the loin were obtained by biopsy in the period 50-60 kg liveweight and analysed for fatty acid composition. In addition, samples of belly fat, the subcutaneous fat and the loin muscle obtained from the carcass were evaluated in terms of fatty acid composition and iodine values. There was no difference between the four experimental groups with regard to the growth performance. Fish oil supplementation increased particularly the concentration of C22:6n-3 (DHA), C20:5n-3 (EPA), and C22:5n-3 (DPA), and addition of coconut oil increased the concentration of the fatty acids C12:0 and C14:0 in the subcutaneous fat and muscle samples. The concentration of these fatty acids was lower in samples obtained from the carcass than from biopsies, but the effect of the dietary oil treatments was still significant at slaughter. Thus, fish oil supplementation increased the level of DHA, EPA, and DPA in muscle and fat tissue, and decreased the n-6/n-3 ratio. However, feeding of 3-6% unrefined fish oil until approximately 60 kg liveweight caused off-flavour of the pork, which was therefore not suitable for human consumption.