Ejaculates, collected over a 35-month period from 3 Polish Landrace boars from 8 months of age, were diluted (30 x 10⁶sperm/cm³) with a standard extender, Kortowo 3 (K3) supplemented with lipoprotein fractions extracted from hen (K3-LPFh) or ostrich egg yolk (K3-LPFo). Thereafter, the diluted sperm samples were stored for 4 days (D0 to D4) at 5° and 16°C, respectively. Irrespective of the extender composition and storage temperatures, sperm motility was gradually reduced during prolonged storage. The decline in sperm motility was more accentuated in K3 extender compared with LPF-based extenders. Seasonal influence was more apparent in K3-LPFo extender during storage at 5°C. The results of this study demonstrate that the inclusion of lipoprotein fractions in semen extender enhanced cold shock resistance of boar spermatozoa, particularly during storage at 5°C.