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ORIGINAL PAPER
Effect of feeding high fat diets differing in fatty acid composition on oxidative stress markers and protein expression in mouse kidney
1
West Pomeranian University of Technology, Faculty of Biotechnology and Animal Husbandry, Department of Physiology, Cytobiology and Proteomics, Szczecin 71-270, Poland
2
The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, Department of Animal Nutrition, 05-110 Jabłonna, Poland
3
Medical University of Warsaw, Centre for Preclinical Research and Technology, Laboratory of Regenerative Medicine, 02-091 Warsaw, Poland
4
Institute of Genetics and Animal Biotechnology of the Polish Academy of Sciences, Department of Genomics and Biodiversity, 05-552 Magdalenka, Poland
Publication date: 2024-02-19
Corresponding author
A. Wypych
West Pomeranian University of Technology, Faculty of Biotechnology and Animal Husbandry, Department of Physiology, Cytobiology and Proteomics, Szczecin 71-270, Poland
West Pomeranian University of Technology, Faculty of Biotechnology and Animal Husbandry, Department of Physiology, Cytobiology and Proteomics, Szczecin 71-270, Poland
J. Anim. Feed Sci. 2024;33(2):170-184
KEYWORDS
TOPICS
ABSTRACT
High-fat diets (HFDs) are associated with the development
of metabolic disorders. However, the type of dietary fat may also be a critical
risk factor. The study aimed to determine the effect of three months of feeding
HFDs with different fatty acid composition on oxidative stress markers and the
proteome of mouse kidneys. The study involved 24 Swiss-Webster mice, which
were divided into four groups (n = 6) fed for three months a standard chow (STD),
an HFD rich in saturated fatty acids (SFA), and HFDs rich in polyunsaturated fatty
acids with linoleic acid to α-linolenic acid ratios of 14:1 (HR) or 5:1 (LR). Feeding
the SFA and HR diets increased triglyceride levels compared to the STD and
LR groups. The HR group also had higher concentrations of thiobarbituric acid
reactive substances compared to the other groups. There was no effect of HFD
on cholesterol concentration and activity of antioxidant enzymes. The SFA diet
increased the expression of acyl-CoA thioesterase 2 and D-lactate dehydrogenase,
while decreasing that of apolipoprotein E. All HFDs led to downregulation of ATP
synthase F1 subunit beta, mitochondrial, while only the LR diet increased the
expression of persulphide dioxygenase ETHE1 and electron transfer flavoprotein
subunit A, suggesting an enhanced oxidation of fatty acids. Ingestion of the SFA
and HR diets caused downregulation of molecular chaperones and upregulation
of the inflammation and apoptosis regulator peptidylprolyl isomerase A. In
conclusion, feeding SFA and HR diets induces oxidative stress and proteome
changes in the mouse kidney. The SFA diet most significantly influenced the
expression pattern of proteins related to energetic metabolism.
FUNDING
This work was financially supported by two sources. The dietary experiment was carried out with the support of the KNOW (Leading National Research Centre) Scientific Consortium “Healthy Animal – Safe Food under the decision of the Ministry of Science and Higher Education No. 05-1/KNOW2/2015, grant No. KNOW2015/CB/PRO1/44. The analyses performed were supported by the Rector of the West Pomeranian University of Technology in Szczecin for PhD students of the Doctoral School, grant number: 35/2022.
CONFLICT OF INTEREST
The Authors declare that there is no conflict of interest.
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