The studies were designed to assess whether a glucose-enriched diet could affect natural and/or PMSG/hCG induced puberty attainment in gilts. In Experiment 1, 145 day old crossbred gilts were randomly allocated to receive a control-standard (n=25) or glucose-supplemented diet (60 g/kg feed; n=25) for 25 days in three equal portions at 8.00, 13.00 and 18.00 h .Gilts were observed morning and afternoon for the detection of oestrus. In Experiment 2, fifty 145-155 day old prepubertal gilts were divided into two equal groups and fed for 25 days as in Experiment 1. On Day 26 of the experiment, all gilts were injected with 750 IU PMSG and 72 h later with 500 IU hCG. Three days after the last injection of gonadotropin, the gilts were slaughtered at a commercial abattoir and their reproductive tracts and carcasses were examined. Based on observations of vulval development and behavioural signs of oestrus, gilts given the glucose-enriched diet in Experiment 1 reached oestrus earlier (170.1±0.9 day) than the control group (175.9±1.6 day; P<0.05). Also gilts given the glucose-supplemented diet showed more intense signs of oestrus than control gilts (2.8±0.4 vs 2.5±0.7 points, respectively; P<0.05). In Experiment 2 three gilts fed the glucose-enriched diet and three fed the control-standard diet did not respond to PMSG/hCG treatment. In the remaining animals glucose supplementation of feed increased ovulation rate (8.5±0.7 vs 6.2±0.6; P<0.05) as well as ovary weight (4.9±0.5 vs 3.6±0.3 g; P<0.05). Similarly, weight (356.5±23.8 vs 264.7±20.2 g) and capacity (723.0±65.8 vs 511.0±35.7 cm³) of uterus were significantly (P<0.01) higher in for gilts fed glucose-enriched diet than for controls. The results show that the addition of glucose to the standard diet stimulated puberty attainment and/or ovarian and reproductive tract receptiveness to PMSG/hCG in prepubertal gilts.