Eight Holstein Red-and-White cows were used in two squares of a 4 x 4 Latin square design experiment and fed for 84 days (weeks 4-16 of lactation) with Ca salts of fatty acids (CaSFA) of linseed oil and animal fat. Experimental diets contained 0, 3, 6 and 9% CaSFA (on D M basis) and vitamin E at doses of 40 and 168 mg/kg DM of concentrate (DM). Cows were fed grass silage and 0.280 kg of concentrate per kg of milk. Average FCM yield was 24.0±0.5 kg/d with 40.0±0.7 and 31.2±0.3 g/kg fat and protein, respectively. No significant effects of CaSFA or vitamin E on feed intake, milk yield, milk fat, protein or lactose contents were received. A tendency towards decreasing protein and increasing milk fat content was observed at a 9% CaSFA in the diet. The addition of CaSFA significantly (P>0.01) elevated UFA (78.07 to 67.06 g/100 g), MUFA (19.44 to 29.18 g/100 g), and PUFA (2.49 to 3.76 g/100 g). DFA (a hypocholesterolemic acid) in milk was increased from 29.35 to 42.59 g/100 g (P<0.01) but total cholesterol decreased from 10.43 to 10.02 mg/100 ml of milk (P>0.05). CaSFA from linseed oil and animal fat significantly decreased the concentration of C₁₀, C₁₂and C₁₄ acids, but slightly increased the concentration of C_18:1, C_18:2, C_18:3n3, C_18:3n6 in the milk (P>0.01). The concentrations of EPA and DHA were not affected by treatments (P>0.05). There were no significant effects of vitamin E on the fatty acid content in milk (P>0.05).