The study aims to investigate the effect of replacing dietary maize with 0, 50, 100, 150 and 200 g/kg of triticale on production performance, egg quality, yolk fatty acids, and blood variables when fed to laying hens. Five experimental diets were mixed to be isoenergetic and isonitrogenous and were fed to the layers for six weeks, from 22 to 28 weeks of age. Three hundred and sixty 22-week-old Hy-Line brown laying hens were individually weighed and randomly allocated to 60 enriched cages giving six birds per cage, in an environmentally controlled experimental house. The cages were equipped with a laying nest, scratching area, metal feeders in front and two nipple drinkers inside. Each diet was fed to 12 cages following randomisation. Feed and water were available ad libitum throughout the experiment. Production variables remained unaffected by the diets containing triticale at various levels. Among the egg quality traits, egg yolk colour linearly decreased (P < 0.001) by dietary triticale inclusion. Dietary triticale did not have an impact on egg yolk fatty acid composition (P > 0.05). Apart from the glucose level, increasing in a linear fashion (P < 0.001) with triticale in diets, the rest of the studied blood variables did not respond (P > 0.05) to changes in dietary triticale. It has been concluded that 20% replacement of maize with triticale in laying hen diets did not have any detrimental impact on egg production variables or the efficiency of production.