Aims of this study were to evaluate the effect of body weight, age, parity, litter size and temperament on the Lacaune ewes’ milk production (length of lactation (LL), total milk yield (TMY), standard milk yield (milk between the 60th and 150th day of lactation (SMY)) and daily milk yield (DMY)) and the entrance order into the milking parlour. The effect of entrance order (the first vs the last group) on the milk production (LL, TMY, SMY, DMY) and composition from 143 ewes was also analysed. The milking period started on the mid of March, and lasted for 229 days on average, and a two-platform milking parlour was used. Moreover, out of the total number of 143 animals, 22 ewes were selected that entered the parlour as the first group (FG) and 18 ewes – as the last group (LG), in all observations. The order of sheep at the entrance to the milking parlour was observed in four periods. Parity and temperament affected the milk production traits. Furthermore, the ewes’ entrance order was significantly affected by their body weight and temperament. Entrance order into milking parlour did not exert an effect on the LL and milk composition; however, FG had significantly higher TMY, SMY and DMY in comparison to LG as well as daily milk fat and protein yield. These data prove that calmer and heavier ewes entered the milking parlour earlier than other ewes; moreover, a later entrance into the milking parlour may be associated with reduced milk production.