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SHORT COMMUNICATION
Effect of thymosin β on maturation of pig oocytes and quality of in vitro produced embryos
1
National Research Institute of Animal Production, Department of Reproductive Biotechnology and Cryopreservation, 32-083 Balice/Kraków, Poland
Publication date: 2020-11-18
Corresponding author
K. Poniedziałek-Kempny
National Research Institute of Animal Production Department of Reproductive Biotechnology and Cryopreservation, 32-083 Balice/Krakow, Poland
National Research Institute of Animal Production Department of Reproductive Biotechnology and Cryopreservation, 32-083 Balice/Krakow, Poland
J. Anim. Feed Sci. 2020;29(4):358-361
KEYWORDS
TOPICS
ABSTRACT
Thymosin (TMS) is a peptide hormone that has regenerative
qualities. To date, the regenerative qualities of thymosin have been tested
only on somatic cells and bovine oocytes. The objective of this study was
to determine the effect of thymosin on the in vitro maturation of pig oocytes
and on in vitro fertilization (IVF), which would be used to assess the quality of
obtained embryos. Pig oocytes (n = 463) were matured in vitro to the Met II
stage in medium supplemented with TMS. After IVF the zygotes were cultured
in vitro until they reached the blastocyst stage. The number of cells and the
level of nuclear DNA fragmentation were evaluated. The addition of TMS to
the maturation medium resulted in a significant increase in the percentage of
competent oocytes that, after fertilization, developed to the blastocyst stage.
Moreover, supplementing the maturation medium with TMS had a positive effect
on the quality of obtained blastocysts by limiting the occurrence of apoptosis.
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