Three lactating multiparous Sannen dairy goats in late lactation (146±7 days of milk) were used in a 3×3 Latin square design to determine the effects of abomasal infusion of cottonseed oil and dietary enzyme supplementation on milk yield and composition. Treatments were twice daily abomasal infusion of 1. 50 ml d^-1 water plus 5 g kemzyme enzyme kg^-1 dry matter (DM) of feed (ENZ), 2. 50 g cottonseed oil d^-1 (OIL), and 3. 50 g cottonseed oil d^-1 plus 5 g kemzyme enzyme kg^-1 DM of feed (ENZ+OIL). Goats were fed a basal diet of 40% lucerne hay and 60% concentrates offered ad libitum. Each experimental period consisted of 14 days of adaptation and 5 days of total collection. Abomasal infusion of cottonseed oil decreased (P<0.05) DM intake and increased (P<0.05) milk fat percentage. Dietary supplementation of enzyme in the diet increased (P<0.05) DM and organic matter (OM) intake but had no effect (P>0.05) on apparent digestibility of DM, OM, crude protein, acid detergent fibre, neutral detergent fibre and ether extract. Cholesterol and plasma triglycerides increased (P<0.05) with cottonseed oil infusion but infusion of cottonseed oil with dietary enzyme supplementation had no (P>0.05) added benefit on lactational performance of the goats. These results suggest that abomasal infusion of cottonseed oil increased the amount of lipids reaching the mammary tissues and therefore increased milk fat percentage without altering milk yields.