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ORIGINAL PAPER
Effects of dietary lysine levels on jejunal expression of amino
acids transporters and hindgut microflora in weaned pigs
1
Sichuan Agricultural University, Institute of Animal Nutrition, Chengdu, 611130, China
2
Evonik Degussa (China) Co., Ltd. Health & Nutrition, Beijing, 100026, China
3
Evonik Industries AG Health & Nutrition, Hanau-Wolfgang, 63457, Germany
Publication date: 2018-08-24
Corresponding author
J. Anim. Feed Sci. 2018;27(3):238-247
KEYWORDS
TOPICS
ABSTRACT
The objective of the study was to evaluate the effects of increasing
dietary standardized ileal digestible (SID) lysine (Lys) level on the expression
of jejunal amino acids (AAs) transporters and the microflora in the hindgut of
weaned pigs. One hundred and twenty weanling pigs weighing 8.10 ± 0.48 kg
were randomly assigned according to body weight and sex to 5 treatments with
6 replicates per treatment and 4 pigs per replicate. Pigs were fed diets with
0.98, 1.11, 1.23, 1.35 or 1.48% of SID Lys for 28 days. The mRNA expression of
cationic amino acids transporter 1 (CAT1) in jejunum was higher in groups fed
with 1.23, 1.35 and 1.48% SID Lys addition (P < 0.05). There was stated a linear
increase in the mRNA expressions of CAT1, excitatory amino acids carrier 1
(EAAC1) and peptide transporter T1 (PEPT1) (linear, P < 0.05). In the caecum,
the populations of bacteria and the content of butyric acid were significantly
influenced (P < 0.05) by dietary SID Lys. In animals fed diet with 1.35% SID Lys
a content of butyric acid was the highest (P < 0.05). Moreover, the populations
of Lactobacillus and Bifidobacterium in caecum and colon increased (linear,
P < 0.05; quadratic, P < 0.05) as dietary SID Lys level increased. The obtained
results showed that dietary SID Lys level may influence AAs absorption and
promote the hindgut health. The suggested supplementation of SID Lys to the
weaned pigs’ diet is 1.35%.
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