The effect of 6 commercial enzyme preparations, degrading mainly the non-starch polysaccharides, on the nutritional value of low-glucosinolate rapeseed meal (RSM) was evaluated in vitro and in vivo. Solubility of protein, ADF and NDF in RSM incubated with or without the addition of 1% of enzyme was measured in vitro. The protein and amino acid digestibility and metabolizable energy of RSM was determined on 4-weeks old broiler cockerels in a balance experiment, while growth performance was evaluated for 3 weeks in 8-day old cockerels fed diets containing 35% of RSM unsupplemented or supplemented with 0,1 % of enzyme. In vitro protein solubility was increased by Bio-feed Pro and Energex and solubility of NDF and ADF by Energex only after 10 h incubation. None of the preparations improved significantly the protein and amino acids digestibility nor AME_N value in the balance experiment but significant changes in apparent digestibility of NDF and A DF were found. No statistically significant effects of enzymes on chicken performance were noted, however chicks fed the diet with Bio-feed Plus were the heaviest and better utilized feed, while those fed with Bio-feed Pro had lower feed utilization.