Effects of fatty acids on cytosolic TAG accumulation
in primary cultured bovine mammary epithelial cells

We examined the effects of short-, medium- or long-chain fatty acids on the accumulation of cytosolic triacylglycerol (TAG) in bovine mammary epithelial cells (bMEC). Octanoate and longchain fatty acids stimulated TAG accumulation in a concentration-dependent manner from 1 to 10 mM and from 50 to 400 µM, respectively, as well as mRNA expression of CD36 and UCP2. Octanoate, oleate and linoleate increased lipid droplet formation. Leptin mRNA expression was significantly reduced by addition of acetate and butyrate but was elevated by addition of oleate and linoleate. Long-chain fatty acids stimulated αs1-casein mRNA expression.

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1230-1388

Assigning DOI numbers, introducing articles from supplements and recent publications to POL-index database, maintaining anti-plagiarism detection, electronic system to proceed manuscripts and webpage of the Journal with interactive pdf files, and language correction of manuscripts published in Journal of Animal and Feed Sciences are financed in the years 2018–2019 by the Ministry of Science and Higher Education from the funds for science popularization activities, Agreement No. 631/P-DUN/2018.