ORIGINAL PAPER
Effects of supplementation with Angelica sinensis Radix
and its powder formulation on growth performance, serum
antioxidant capacity, immune response and intestinal microflora
of broiler chickens
1
Gansu Academy of Sciences, Institute of Biology, Key Laboratory of Microbial Resources Exploitation
and Application of Gansu Province, Lanzhou 730000, China
Publication date: 2024-08-27
Corresponding author
Z. Y. Wang
Gansu Academy of Sciences, Institute of Biology, Key Laboratory of Microbial Resources Exploitation and Application of Gansu Province, Lanzhou 730000, China
Gansu Academy of Sciences, Institute of Biology, Key Laboratory of Microbial Resources Exploitation and Application of Gansu Province, Lanzhou 730000, China
KEYWORDS
TOPICS
ABSTRACT
The objective of this study was to characterise the effects of supplementation
with Angelica sinensis Radix (ASR) powder and a preparation containing
ASR powder (Danggui Buxue Decoction, DBD) on growth performance,
antioxidant capacity, immune activity, and caecal bacterial microbiota in broiler
chickens. A total of 180 one-day-old male Luhua broiler chickens were randomly
assigned to three groups. Broiler chickens in the control group were fed a basal
diet, the ASR group was administered a basal diet supplemented with 1% ASR
powder, and the DBD group received a basal diet supplemented with 1% DBD
powder. Results showed significant increases in serum glutathione peroxidase
and catalase activities, as well as serum immunoglobulin A levels in both the
ASR and DBD groups compared to the control group (P < 0.05). Additionally,
the malondialdehyde content was significantly lower in the ASR group compared
to other groups (P < 0.05). Supplementation with ASR and DBD also had
notable effects on the composition of broiler intestinal microbiota, particularly
influencing the abundance of Firmicutes and Bacteroidetes, as demonstrated by
high-throughput sequencing analysis. These findings suggest that dietary ASR
and DBD supplementation enhanced antioxidant capacity, immune status, and
intestinal flora diversity in broiler chickens, indicating their potential as effective
phytogenic feed additives.
FUNDING
The presented work was financed by ‘Gansu Top Leading Talent Plan, 2021’ and ‘The Youth Fund of Gansu Academy of Sciences, grant number 2023QN-04’.
CONFLICT OF INTEREST
The Authors declare that there is no conflict of interest.
REFERENCES (57)
1.
Abd El-Hack M.E., El-Saadony M.T., Salem H.M. et al., 2022. Alternatives to antibiotics for organic poultry production: types, modes of action and impacts on bird's health and production. Poult. Sci. 101, 101696, https://doi.org/10.1016/j.psj.....
2.
Bajagai Y.S., Petranyi F., Yu S.J., Lobo E., Batacan R., Kayal A., Horyanto D., Ren X., Whitton M.M., Stanley D., 2022. Phytogenic supplement containing menthol, carvacrol and carvone ameliorates gut microbiota and production performance of commercial layers. Sci. Rep. 12, 11033, https://doi.org/10.1038/s41598....
3.
Bhattacharyya A., Chattopadhyay R., Mitra S., Crowe S.E., 2014. Oxidative stress: an essential factor in the pathogenesis of gastrointestinal mucosal diseases. Physiol. Rev. 94, 329–354, https://doi.org/10.1152/physre....
4.
Breedveld A., van Egmond M., 2019. IgA and FcαRI: Pathological roles and therapeutic opportunities. Front Immunol. 10, 553, https://doi.org/10.3389/fimmu.....
5.
Cantarel B.L., Lombard V., Henrissat B., 2012. Complex carbohydrate utilization by the healthy human microbiome. PLoS One 7, e28742, https://doi.org/10.1371/journa....
6.
Carsetti R., Rosado M.M., Wardmann H., 2004. Peripheral development of B cells in mouse and man. Immunol Rev. 197, 179–191, https://doi.org/10.1111/j.0105....
7.
Chang W.C., Yen C.C., Cheng C.P., Wu Y.T., Hsu M.C., 2020. Chinese herbal decoction (Danggui Buxue Tang) supplementation augments physical performance and facilitates physiological adaptations in swimming rats. Pharm. Biol. 58, 545–552, https://doi.org/10.1080/138802....
8.
Chen C.Y., Li H., Yuan Y.N., Dai H.Q., Yang B., 2013a. Antioxidant activity and components of a traditional Chinese medicine formula consisting of Crataegus pinnatifida and Salvia miltiorrhiza. BMC Complement. Med. Ther. 13, 99, https://doi.org/10.1186/1472-6....
9.
Chen H., Chen X., Ping Z.L., Jiang X.W., Ge M., Ma J., Yu W.H., 2022. Promotion effect of angelica sinensis extract on angiogenesis of chicken preovulatory follicles in vitro. Poult. Sci. 101, 101938, https://doi.org/10.1016/j.psj.....
10.
Chen X.P., Li W., Xiao X.F., Zhang L.L., Liu C.X., 2013b. Phytochemical and pharmacological studies on Radix Angelica sinensis. Chin. J. Nat. Med. 11, 577–587, https://doi.org/10.1016/S1875-....
11.
Chen Y.R., Zheng H.M., Zhang G.X., Chen F.L., Chen L.D., Yang Z.C., 2020. High Oscillospira abundance indicates constipation and low BMI in the Guangdong Gut Microbiome Project. Sci. Rep. 10, 9364, https://doi.org/10.1038/s41598....
12.
Clark B., Panzone L.A., Stewart G.B., Kyriazakis I., Niemi J.K., Latvala T., Tranter R., Jones P., Frewer L.J., 2019. Consumer attitudes towards production diseases in intensive production systems. PLoS One 14, e0210432, https://doi.org/10.1371/journa....
13.
Du K.H., Wang L., Wang Z.L., Xiao H.X.Z., Hou J.Y., Hu L., Fan N.K., Wang Y.P., 2023. Angelica Sinensis polysaccharide antagonizes 5-Fluorouracil-induced spleen injury and dysfunction by suppressing oxidative stress and apoptosis. Biomed. Pharmacother. 162, 114602, https://doi.org/10.1016/j.biop....
14.
Fan Q., Yang R.J., Yang F.X., Xia P.F., Zhao L., 2020. Spectrum-effect relationship between HPLC fingerprints and antioxidant activity of Angelica sinensis. Biomed. Chromatogr. 34, e4707, https://doi.org/10.1002/bmc.47....
15.
Fan Y., Pedersen O., 2021. Gut microbiota in human metabolic health and disease. Nat. Rev. Microbiol. 19, 55–71, https://doi.org/10.1038/s41579....
16.
Gadde U., Kim W.H., Oh S.T., Lillehoj H.S., 2017. Alternatives to antibiotics for maximizing growth performance and feed efficiency in poultry: a review. Anim. Health Res. Rev. 18, 26–45, https://doi.org/10.1017/s14662....
17.
Gao L.M., Chen X.Y., Fu Z.Y., Yin J., Wang Y.F., Sun W.G., Ren H., Zhang Y.H., 2021. Kinsenoside alleviates alcoholic liver injury by reducing oxidative stress, inhibiting endoplasmic reticulum stress, and regulating AMPK-dependent autophagy. Front Pharmacol. 12, 747325, https://doi.org/10.3389/fphar.....
18.
Gao M., Zhang J.H., Zhou F.X., Xie C.H., Fang S.Q., Zhou Y.F., 2011. Angelica sinensis suppresses human lung adenocarcinoma A549 cell metastasis by regulating MMPs/TIMPs and TGF-β1. Oncol. Rep. 27, 585–593, https://doi.org/10.3892/or.201....
19.
Gilbert J.A., Blaser M.J., Caporaso J.G., Jansson J.K., Lynch S.V., Knight R., 2018. Current understanding of the human microbiome. Nat. Med. 24, 392–400, https://doi.org/10.1038/nm.451....
20.
Gu P.F., Wusiman A., Wang S.Y., Zhang Y., Liu Z.G., Hu Y.L., Liu J.G., Wang D.Y., 2019. Polyethylenimine-coated PLGA nanoparticles-encapsulated Angelica sinensis polysaccharide as an adjuvant to enhance immune responses. Carbohydr. Polym. 223, 115128, https://doi.org/10.1016/j.carb....
21.
Gu Y.Q., Wang T.H., Chen J., Zhou Z., Wang Y., Chen J.J, Liu N., Jiang Z.X., 2020. The Chinese Herb Codonopsis pilosula isolate isorhapontigenin protects against oxidative stress injury by inhibiting the activation of PI3K/Akt signaling pathway. J. Integr. Neurosci. 19, 333–340, https://doi.org/10.31083/j.jin....
22.
Gulcin I., 2020. Antioxidants and antioxidant methods: an updated overview. Arch. Toxicol. 94, 651–715, https://doi.org/10.1007/s00204....
23.
Han Y.S., Xu X., Wang J.X., Cai H.Y., Li D.J., Zhang H.W., Yang P.L., Meng K., 2023. Dietary Bacillus licheniformis shapes the foregut microbiota, improving nutrient digestibility and intestinal health in broiler chickens. Front. Microbiol. 14, 1113072, https://doi.org/10.3389/fmicb.....
24.
Hollister E.B., Gao C.X., Versalovic J., 2014. Compositional and functional features of the gastrointestinal microbiome and their effects on human health. Gastroenterology 146, 1449–1458, https://doi.org/10.1053/j.gast....
25.
Jin M.L., Huang Q.S., Zhao K., Shang P., 2013. Biological activities and potential health benefit effects of polysaccharides isolated from Lycium barbarum L. Int. J. Biol. Macromol. 54, 16–23, https://doi.org/10.1016/j.ijbi....
26.
Kers J.G., Velkers F.C., Fischer E.A.J., Stegeman J.A., Smidt H., Hermes G.D.A., 2022. Conserved developmental trajectories of the cecal microbiota of broiler chickens in a field study. FEMS Microbiol Ecol. 98, https://doi.org/10.1093/femsec....
27.
Kong F.L., Hua Y.T., Zeng B., Ning R.H., Li Y., Zhao J.C., 2016. Gut microbiota signatures of longevity. Curr. Biol. 26, R832–R833, https://doi.org/10.1016/j.cub.....
28.
Lauridsen C., 2019. From oxidative stress to inflammation: redox balance and immune system. Poult. Sci. 98, 4240–4246, https://doi.org/10.3382/ps/pey....
29.
Li C.Y., Zhu F.L., Wang S.S., Wang J., Wu B., 2021. Danggui buxue decoction ameliorates inflammatory bowel disease by improving inflammation and rebuilding intestinal mucosal barrier. Evid. Based Complement. Alternat. Med. 2021, 8853141, https://doi.org/10.1155/2021/8....
30.
Li X.T., Wang B., Li J.L., Yang R., Li S.C., Zhang M., Huang W., Cao L., 2013. Effects of Dangguibuxue Tang, a Chinese herbal medicine, on growth performance and immune responses in broiler chicks. Biolog. Res. 46, 183–188, https://doi.org/10.4067/s0716-....
31.
Lin J., Comi M., Vera P., Alessandro A., Qiu K., Wang J., Wu S.G., Qi G.H., Zhang H.J., 2023. Effects of Saccharomyces cerevisiae hydrolysate on growth performance, immunity function, and intestinal health in broilers. Poult. Sci. 102, 102237, https://doi.org/10.1016/j.psj.....
32.
Litvak Y., Byndloss M.X., Tsolis R.M., Baumler A.J., 2017. Dysbiotic Proteobacteria expansion: a microbial signature of epithelial dysfunction. Curr. Opin. Microbiol. 39, 1–6, https://doi.org/10.1016/j.mib.....
33.
Liu S., Wang K.J., Lin S.Z. et al., 2023. Comparison of the effects between tannins extracted from different natural plants on growth performance, antioxidant capacity, immunity, and intestinal flora of broiler chickens. Antioxidants 12, 441, https://doi.org/10.3390/antiox....
34.
Ma J.P., Guo Z.B., Jin L., Li Y.D., 2015. Phytochemical progress made in investigations of Angelica sinensis (Oliv.) Diels. Chin. J. Nat. Med. 13, 241–249, https://doi.org/10.1016/S1875-....
35.
Mantis N.J., Rol N., Corthesy B., 2011. Secretory IgA's complex roles in immunity and mucosal homeostasis in the gut. Mucosal Immunol. 4, 603–611, https://doi.org/10.1038/mi.201....
36.
Mkaddem S.B., Christou I., Rossato E., Berthelot L., Lehuen A., Monteiro R.C., 2014. IgA, IgA receptors, and their anti-inflammatory properties. Curr. Top. Microbiol. Immunol. 382, 221–235, https://doi.org/10.1007/978-3-....
37.
Nai J.J., Zhang C., Shao H.L., Li B.Q., Li H., Gao L., Dai M.M., Zhu L.Q., Sheng H.G., 2021. Extraction, structure, pharmacological activities and drug carrier applications of Angelica sinensis polysaccharide. Int. J. Biol. Macromol. 183, 2337–2353, https://doi.org/10.1016/j.ijbi....
38.
Oladeinde A., Cook K., Lakin S.M. et al., 2019. Horizontal gene transfer and acquired antibiotic resistance in Salmonella enterica Serovar heidelberg following in vitro incubation in broiler ceca. Appl. Environ. Microbiol. 85, e01903–e01919, https://doi.org/10.1128/AEM.01....
39.
Parker B.J., Wearsch P.A., Veloo A.C.M., Rodriguez-Palacios A., 2020. The genus Alistipes: gut bacteria with emerging implications to inflammation, cancer, and mental health. Front. Immunol. 11, 906, https://doi.org/10.3389/fimmu.....
40.
Pisoschi A.M., Pop A., Cimpeanu C., Predoi G., 2016. Antioxidant capacity determination in plants and plant-derived products: A review. Oxid. Med. Cell Longev. 2016, 9130976, https://doi.org/10.1155/2016/9....
41.
Salim H.M., Huque K.S., Kamaruddin K.M., Beg M., 2018. Global restriction of using antibiotic growth promoters and alternative strategies in poultry production. Sci. Prog. 101, 52–75, https://doi.org/10.3184/003685....
42.
Silva L., Pezzini B., Soares L., 2015. Spectrophotometric determination of the total flavonoid content in Ocimum basilicum L. (Lamiaceae) leaves. Pharmacognosy Magazine. 11, 96–101, https://doi.org/10.4103/0973-1....
43.
Tian Y.H., Zhang J.Y., Li F.H, Wang A.G., Yang Z.Q., Li J.X., 2023. Dietary supplementation with different alternative to in-feed antibiotic improves growth performance of broilers during specific phases. Poult. Sci. 102, 102919, https://doi.org/10.1016/j.psj.....
44.
Turnbaugh P.J., Ley R.E., Mahowald M.A., Magrini V., Mardis E.R., Gordon J.I., 2006. An obesity-associated gut microbiome with increased capacity for energy harvest. Nature. 444, 1027–1031, https://doi.org/10.1038/nature....
45.
Wang C.H., Wu W.J., Su L.Y., Lu C.W., Wang P.H., Lee M.C., Chuang W.C., Wang S.E., Wu C.H., 2021. Chinese herbal medicine formula Guizhi Li-Zhong tang as an alternative to antibiotic feed additives for preventing pneumonia in piglets through antioxidant, anti-inflammatory, and antiapoptotic protection. Evid. Based Complement. Alternat. Med. 2021, 4978783, https://doi.org/10.1155/2021/4....
46.
Wang L.H., Fu G.H., Liu S.Z., Li L., Zhao X., 2019. Effects of oxygen levels and a Lactobacillus plantarum strain on mortality and immune response of chickens at high altitude. Sci. Rep. 9, 16037, https://doi.org/10.1038/s41598....
47.
Wang Y., Wang H.F., Wang B.W., Zhang B.B., Li W.L., 2020. Effects of manganese and Bacillus subtilis on the reproductive performance, egg quality, antioxidant capacity, and gut microbiota of breeding geese during laying period. Poult. Sci. 99, 6196–6204, https://doi.org/10.1016/j.psj.....
48.
Waters J.L., Ley R.E., 2019. The human gut bacteria Christensenellaceae are widespread, heritable, and associated with health. BMC Biol. 17, 83, https://doi.org/10.1186/s12915....
49.
Wei S., Morrison M., Yu Z., 2013. Bacterial census of poultry intestinal microbiome. Poult Sci. 92, 671–683, https://doi.org/10.3382/ps.201....
50.
Wei W.L., Zeng R., Gu C.M., Qu Y., Huang L.F., 2016. Angelica sinensis in China-A review of botanical profile, ethnopharmacology, phytochemistry and chemical analysis. J. Ethnopharmacol. 190, 116–141, https://doi.org/10.1016/j.jep.....
51.
Wu F.L., Ji P., Hu Y.H., Li C.C., He J., 2023. Study on the hepatoprotective effect mechanism of polysaccharides from charred Angelica sinensis on the layer chickens based on the detection of the intestinal floras and short-chain fatty acids of cecal contents and association analysis. Vet. Sci. 10, 224, https://doi.org/10.3390/vetsci....
52.
Wu F.l., Hu Y.h., Ji P., Li C.C., He J., 2022a. Metabonomics study on the hepatoprotective effect mechanism of polysaccharides from different processed products of Angelica sinensis on layer chickens based on UPLC–Q/TOF–MS/MS, multivariate statistical analysis and conjoint analysis. Biomed. Chromatogr. 36, e5362, https://doi.org/10.1002/bmc.53....
53.
Wu S.J., 2018. Effect of dietary Astragalus membranaceus polysaccharide on the growth performance and immunity of juvenile broilers. Poult. Sci. 97, 3489–3493, https://doi.org/10.3382/ps/pey....
54.
Wu Y., Wu C.Y., Che Y.Y. et al., 2022b. Effects of Glycyrrhiza polysaccharides on chickens' intestinal health and homeostasis. Front. Vet. Sci. 9, 891429, https://doi.org/10.3389/fvets.....
55.
Yan F., Wang B.B., Zhang Y., 2014. Polysaccharides from Cordyceps sinensis mycelium ameliorate exhaustive swimming exercise-induced oxidative stress. Pharm. Biol. 52, 157–161, https://doi.org/10.3109/138802....
56.
Zhu Y.Z., Huang S.H., Tan B.K., Sun J., Whiteman M., Zhu Y.C., 2004. Antioxidants in Chinese herbal medicines: a biochemical perspective. Nat. Prod. Rep. 21, 478–489, https://doi.org/10.1039/b30482....
57.
Zhuang C., Xu N.W., Gao G.M., Ni S., Miao K.S., Li C.K., Wang L.M., Xie H.G., 2016. Polysaccharide from Angelica sinensis protects chondrocytes from H2O2-induced apoptosis through its antioxidant effects in vitro. Int. J. Biol. Macromol. 87, 322–328, https://doi.org/10.1016/j.ijbi....
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