ORIGINAL PAPER
Effects of supplementing malate and yeast culture
(Saccharomyces cerevisiae) on the rumen enzyme
profile and growth performance of lambs
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Shahrekord University, Animal Science Department, Agricultural College,
P.O. Box 115, Shahrekord, Iran
Publication date: 2009-03-20
Corresponding author
A. Moharrery
Shahrekord University, Animal Science Department, Agricultural College,
P.O. Box 115, Shahrekord, Iran
J. Anim. Feed Sci. 2009;18(2):283-295
KEYWORDS
ABSTRACT
The objectives of this study were to determine the effects of adding a mixture of malate and yeast
culture (Saccharomyces cerevisiae) on cellulolytic, amylolytic, proteolytic and ureolytic activity in
the rumen and on the growth performance of lambs. Eight native breed (Lory-Bakhtiary) lambs aged
approximately 7 months (four males and four females) were divided into two groups by sex. They
were kept in individual pens and fed a total mixed ration (TMR) based on lucerne hay-wheat straw
and concentrate. The two groups of lambs were randomly assigned to each of the dietary treatments
in a cross-over design: control (without supplementation) and supplemented (with malate and yeast
included in the TMR at a level of 10 g ·kg-1). The experiment was divided into 2 periods, in which the
lambs received one diet in the first three-week period, after which they were transferred to the other
diet for another three weeks. Urease, cellulase, protease and amylase activities were determined in
rumen fluid and rumen microbial biomass. Rumen samples were taken 3-4 h after feeding at the end
of each period. The weights of lambs were recorded at the beginning and end of each period. RNA
equivalents (RNA-e) in rumen fluid and urine were measured for determination of microbial status
in different samples. Supplementation with the malate-yeast mixture increased daily weight gain
(from 196 in controls to 259 g/d) but did not affect (P>0.05) feed intake (0.970 vs 0.929 kg DM/d
for the control vs the supplemented lambs, respectively). Higher cellulase activity was observed in
lambs on the malate-yeast diet compared with the control lamb diet. A significant but moderately
positive correlation was found between cellulase and protease activities (r=0.5188; P=0.0395). No
significant differences were observed between the two treatments for dry matter, neutral detergent
fibre and crude protein apparent digestibilities (P>0.05). Neither was microbial biomass (based on
RNA-e concentrations) affected by the malate-yeast treatments (P>0.05).
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