ORIGINAL PAPER
Evaluation of feed supplementation using legume leaf meal and spent substrate fermented by Eupenicillium javanicum as a potential feed for ruminants: an in vitro study
1
National Research and Innovation Agency, Organization Research for Agriculture and Food, Research Center for Animal Husbandry, 16915 Bogor, Indonesia
Publication date: 2024-08-21
Corresponding author
A. Herliatika
National Research and Innovation Agency, Organization Research for Agriculture and Food, Research Center for Animal Husbandry, 16915 Bogor, Indonesia
National Research and Innovation Agency, Organization Research for Agriculture and Food, Research Center for Animal Husbandry, 16915 Bogor, Indonesia
KEYWORDS
TOPICS
ABSTRACT
Legume forage contains anti-nutrients that mitigate enteric methane production by reducing microbial activity in the rumen. Therefore, this study aimed to observe the inclusion of 49.6% legumes in concentrate, with or without Eupennicillum javanicum-fermented spent substrate supplementation on in vitro feed fermentation and methane emission. The experimental diet consisted of a legume-containing (L) or non-legume (NL) concentrate with (+S) or without (−S) spent substrate at 10% dry matter concentrate. This study used a complete randomised design in a 2 × 2 factorial arrangement with five replicates per treatment. The ratio of concentrate to forage was 1:3, with the legume component containing a mixture of Indigofera sp. and Gliricidia sp. at a ratio of 3:1. The results showed that Indigofera sp. and Gliricidia sp. could substitute 49.6% of the concentrate without affecting feed digestibility. Furthermore, the addition of Indigofera sp., Gliricidia sp., and spent substrate effectively reduced methane production in ruminants.
ACKNOWLEDGEMENTS
The authors are grateful to the Government of Indonesia for providing financial support for this project through the revenue and expenditure budget [2021], under Research Project Number: 4585.SDA.502/051/C/C2/APBN/2021.
CONFLICT OF INTEREST
The Authors declare that there is no conflict of interest.
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