ORIGINAL PAPER
Fermentation quality of lucerne laboratory
silage treated with Clostridium cellobioparum,
formic acid and Lactomix
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1
Institute of Genetics and Animal Breeding, Polish Academy of Sciences,
Jastrzebiec, 05-551 Mroków, Poland
2
Agricultural University of Warsaw,
Rakowiecka 26/28, 02-525 Warszawa, Poland
Publication date: 1994-02-21
J. Anim. Feed Sci. 1994;3(1):65-72
KEYWORDS
ABSTRACT
The experiment was designed to compare and evaluate the effect of the addition of
C. cellobioparum bacteria, or other standard additives, to lucerne silage noting the resulting quality.
The first cut of direct-cut lucerne was ensiled in laboratory scale and treated as follows: I. untreated
(control), II. formic acid (FA) 80% - 2 ml kg-1, III. Clostridium cellobioparum live cellulolytic bacteria
culture added in a water suspension at the rate of 10 ml kg (Cclb) - 1x 106 g-1,
IV. Clostridium cellobioparum bacteria culture - 1x106 g-1 live cellulolytic bacterial culture added
in a water suspension at the rate of 10 ml/kg (Cclb) and Lactomix (LAC) - 1.5 g kg-1, V. Lactomix
- 1.5 g kg-1.
Following an eight-week storage time the results were analyzed. FA treated silage was well
preserved, with a pH of 4.10 and protein degradation rate of 75.4 g ammonia N kg-1 total N. The
fermentation parameters of the Cclb inoculated silage were within an acceptable range, with a pH of
4.17 and degradation rate of 102.6 g ammonia N kg-1 total N . The ammonia levels of LAC inoculated
silage were found to be 119.5 g ammonia N kg-1 total N. Hence, the Cclb was found to have better
fermentation quality than the LAC-inoculated samples, but slightly lower than the FA treatment.