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SHORT COMMUNICATION
Gene expression in bovine mammary somatic cells
isolated from milk
1
Nutrition and Behaviour Group, AgResearch Limited
2
Institute of Food, Nutrition and Human Health, Massey University,
Palmerston North, New Zealand
Publication date: 2004-08-30
J. Anim. Feed Sci. 2004;13(Suppl. 1):401-404
KEYWORDS
ABSTRACT
Traditionally, in order to examine the expression of genes involved in mammary gland
metabolism, RNA was prepared from mammary biopsies or from tissue samples taken at slaughter.
The objective of this study was to validate the use of milk somatic cells as a model to study gene
expression in the lactating bovine mammary gland. The expression pattern of milk protein and
epithelial cell marker genes was similar between somatic cells and mammary tissue indicating that
cells exfoliated into milk provide a viable non-invasive tissue source for gene profiling in the bovine
mammary gland.
CITATIONS (8):
1.
Evaluation of a replacement method for mammary gland biopsies by comparing gene expression in udder tissue and mammary epithelial cells isolated from milk
K. Krappmann, R. Weikard, C. Kühn
Research in Veterinary Science
K. Krappmann, R. Weikard, C. Kühn
Research in Veterinary Science
2.
Use of milk epithelial cells to study regulation of cell activity and apoptosis during once-daily milking in goats
H. Ben Chedly, P. Lacasse, P.-G. Marnet, M. Komara, S. Marion, M. Boutinaud
animal
H. Ben Chedly, P. Lacasse, P.-G. Marnet, M. Komara, S. Marion, M. Boutinaud
animal
3.
Expression Profiling of Glucose Transporter 1 (GLUT1) and Apoptotic Genes (BAX and BCL2) in Milk Enriched Mammary Epithelial Cells (MEC) in Riverine Buffalo during Lactation
Poonam Yadav, Desh Deepak Singh, Manishi Mukesh, R. S. Kataria, Anita Yadav, A. K. Mohanty, B. P. Mishra
Animal Biotechnology
Poonam Yadav, Desh Deepak Singh, Manishi Mukesh, R. S. Kataria, Anita Yadav, A. K. Mohanty, B. P. Mishra
Animal Biotechnology
4.
Unilateral once daily milking locally induces differential gene expression in both mammary tissue and milk epithelial cells revealing mammary remodeling
Marion Boutinaud, Laurent Galio, Vanessa Lollivier, Laurence Finot, Sandra Wiart, Diane Esquerré, Eve Devinoy
Physiological Genomics
Marion Boutinaud, Laurent Galio, Vanessa Lollivier, Laurence Finot, Sandra Wiart, Diane Esquerré, Eve Devinoy
Physiological Genomics
5.
Maintaining RNA integrity in a homogeneous population of mammary epithelial cells isolated by Laser Capture Microdissection
Claudia Bevilacqua, Samira Makhzami, Jean-Christophe Helbling, Pierre Defrenaix, Patrice Martin
BMC Cell Biology
Claudia Bevilacqua, Samira Makhzami, Jean-Christophe Helbling, Pierre Defrenaix, Patrice Martin
BMC Cell Biology
6.
Milking and Feed Restriction Regulate Transcripts of Mammary Epithelial Cells Purified from Milk
M. Boutinaud, M.H. Ben Chedly, E. Delamaire, J. Guinard-Flament
Journal of Dairy Science
M. Boutinaud, M.H. Ben Chedly, E. Delamaire, J. Guinard-Flament
Journal of Dairy Science
7.
The decrease in milk yield during once daily milking is due to regulation of synthetic activity rather than apoptosis of mammary epithelial cells in goats
Chedly Ben, P. Lacasse, P-G. Marnet, M. Boutinaud
animal
Chedly Ben, P. Lacasse, P-G. Marnet, M. Boutinaud
animal
8.
Sodium Butyrate More Effectively Mitigates the Negative Effects of High-Concentrate Diet in Dairy Cows than Sodium β-Hydroxybutyrate via Reducing Free Bacterial Cell Wall Components in Rumen Fluid and Plasma
Yongjiang Wu, Yawang Sun, Ruiming Zhang, Tianle He, Guohao Huang, Ke Tian, Junhui Liu, Juncai Chen, Guozhong Dong
Toxins
Yongjiang Wu, Yawang Sun, Ruiming Zhang, Tianle He, Guohao Huang, Ke Tian, Junhui Liu, Juncai Chen, Guozhong Dong
Toxins
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| ISSN: | 1230-1388 |
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