The aim of the study was to evaluate the effect of dietary inclusion of crude glycerine on growth performance, carcass traits, body measurements and blood cholesterol level of growing Japanese quails. In total 375 one-dayold Japanese quails were randomly divided into five equal groups under complete randomized design. Experimental groups were as follows: T1: basal diet (without glycerine addition), T2: basal diet + 2.5% glycerine, T3: basal diet + 5% glycerine, T4: basal diet + 7.5% glycerine, and T5: basal diet + 10% glycerine. Each treatment was replicated 5 times, 15 birds in each replicate. It was observed that glycerine supplementation did not significantly (P > 0.05) affect the feed intake and body weight of birds. Dietary inclusion of glycerine at a dose of 2.5 and 5% had positive impact on feed conversion ratio (quadratic, P = 0.021) in comparison to control group. Glycerine inclusion in the diet did not affect total blood cholesterol level. Furthermore, neither carcass traits nor body measurements were statistically influenced by the different inclusion levels of glycerine. So, it could be stated that in order to obtain positive effects on quail growth performance, crude glycerine could be used in diets as a source of energy at levels of 2.5 and 5%