Improving the analysis of fatty acids using a
combination of gas chromatography and Ag +  liquid
chromatography

A method is proposed for routine determination of fatty acids (FAs), including conjugated fatty acids, in the liver of rats and sheep using gas-liquid chromatography (GLC) and Ag⁺-HPLC. The proposed procedure includes new methods for saponification carried out at room temperature and extraction of free FAs. The obtained free FAs are methylated in an acid-base catalyzed procedure. Due to simultaneous fractionation of methylated FAs using GLC and Ag⁺-HPLC, the accuracy and sensitivity of the procedure is improved (especially for conjugated linoleic acids).

CITATIONS (3):

CrossRef

Submit your paper

Instructions for Authors

Archive

Send by email

Content of orotic acid and selected bioactive compounds in ovine milk during lamb rearing

Improved lipid saponification for chromatographic quantification of fatty acids in porcine erythrocytes – an important lipidomic biomarker of the effectiveness of dietary fat supplementation in pigs as a large animal model for human studies

Is royal jelly a sustainable alternative lipid source in aquaculture? Influence of dietary royal jelly levels on fatty acid composition in zebrafish

Enrichment of lamb rations with carnosic acid and seleno-compounds affects the content of selected lipids and tocopherols in the pancreas

Effect of grazing on composition, fatty acid profile and nutritional indices of the goat milk and cheese

Indexes

Keywords index

Topics index

Authors index

Assigning DOI numbers, introducing articles from supplements and recent publications to POL-index database, maintaining anti-plagiarism detection, electronic system to proceed manuscripts and webpage of the Journal with interactive pdf files, and language correction of manuscripts published in Journal of Animal and Feed Sciences are financed in the years 2018–2019 by the Ministry of Science and Higher Education from the funds for science popularization activities, Agreement No. 631/P-DUN/2018.

We process personal data collected when visiting the website. The function of obtaining information about users and their behavior is carried out by voluntarily entered information in forms and saving cookies in end devices. Data, including cookies, are used to provide services, improve the user experience and to analyze the traffic in accordance with the Privacy policy. Data are also collected and processed by Google Analytics tool (more).

You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.

I agree I do not agree