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SHORT COMMUNICATION
Improving the analysis of fatty acids using a
combination of gas chromatography and Ag+ liquid
chromatography
1
The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences,
05-110 Jabłonna, Poland
Publication date: 2005-06-26
Corresponding author
M. Czauderna
The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jabłonna, Poland
The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jabłonna, Poland
J. Anim. Feed Sci. 2005;14(Suppl. 1):563-566
KEYWORDS
ABSTRACT
A method is proposed for routine determination of fatty acids (FAs), including conjugated fatty
acids, in the liver of rats and sheep using gas-liquid chromatography (GLC) and Ag+-HPLC. The
proposed procedure includes new methods for saponification carried out at room temperature and
extraction of free FAs. The obtained free FAs are methylated in an acid-base catalyzed procedure.
Due to simultaneous fractionation of methylated FAs using GLC and Ag+-HPLC, the accuracy and
sensitivity of the procedure is improved (especially for conjugated linoleic acids).
CITATIONS (3):
1.
Effect of dietary conjugated linoleic acid and selenized yeast on the concentration of fatty acids and minerals in rats
Marian Czauderna, Jan Kowalczyk, Katarzyna Korniluk
Archives of Animal Nutrition
Marian Czauderna, Jan Kowalczyk, Katarzyna Korniluk
Archives of Animal Nutrition
2.
The analysis of lamb and beef fatty acids with a 30 m BPX70 column is comparable with a 120 m column
Edward H. Clayton, Benjamin W. B. Holman, Richard G. Meyer
Edward H. Clayton, Benjamin W. B. Holman, Richard G. Meyer
3.
The analysis of lamb and beef fatty acids with a 30 m BPX70 column is comparable with a 120 m column
Edward H Clayton, Benjamin W.B. Holman, Richard G Meyer
Journal of Food Composition and Analysis
Edward H Clayton, Benjamin W.B. Holman, Richard G Meyer
Journal of Food Composition and Analysis
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| ISSN: | 1230-1388 |
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