ORIGINAL PAPER
Intestinal nitrogen flow, total nitrogen and 15N balance in pigs labelled intravenously with 15N-leucine and fed a meat meal diet
 
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1
Research Institute for the Biology of Farm Animals, Department of Nutritional Physiology "Oskar Kellner", 0-2500 Rostock, Germany
 
2
Institute of Animal Nutrition, University of Rostock, 0-2500 Rostock, Germany
 
3
Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jabłonna, Poland
 
 
Publication date: 1995-08-22
 
 
J. Anim. Feed Sci. 1995;4(3):207-215
 
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ABSTRACT
Three pigs of about 33 kg body weight, each fitted with re-entrant cannulas into the duodenum and ileum and with bladder catheters were used for the study. One of the pigs was infused intravenously with 15N-leucine for 8 days. Total N passage, 15N nitrogen passage through the duodenum and ileum, and N balance were determined. The mean amount of total N passing the duodenum exceeded the ingested N by 12%, and that passing the ileum accounted for 27% of N intake. Of the infused 15N, 67.4% was found in the urine, faeces, digesta and carcass after slaughter. Taking the recovered amount of 15N as 100%, 20.4% was found in urine, 0.6% in faeces, 8.2% in digesta and 70.8% in tissues. It was concluded that 15N retention from intravenously administrated leucine was much higher than from 15N in urea given with feed, as estimated previously.
 
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ISSN:1230-1388
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