ORIGINAL PAPER
Intestinal nitrogen flow, total nitrogen and
15N balance in pigs labelled intravenously with
15N-leucine and fed a meat meal diet
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1
Research Institute for the Biology of Farm Animals,
Department of Nutritional Physiology "Oskar Kellner",
0-2500 Rostock, Germany
2
Institute of Animal Nutrition, University of Rostock,
0-2500 Rostock, Germany
3
Kielanowski Institute of Animal Physiology and Nutrition,
Polish Academy of Sciences,
05-110 Jabłonna, Poland
Publication date: 1995-08-22
J. Anim. Feed Sci. 1995;4(3):207-215
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ABSTRACT
Three pigs of about 33 kg body weight, each fitted with re-entrant cannulas into the duodenum
and ileum and with bladder catheters were used for the study. One of the pigs was infused
intravenously with 15N-leucine for 8 days. Total N passage, 15N nitrogen passage through the
duodenum and ileum, and N balance were determined. The mean amount of total N passing the
duodenum exceeded the ingested N by 12%, and that passing the ileum accounted for 27% of
N intake. Of the infused 15N, 67.4% was found in the urine, faeces, digesta and carcass after slaughter.
Taking the recovered amount of 15N as 100%, 20.4% was found in urine, 0.6% in faeces, 8.2% in
digesta and 70.8% in tissues. It was concluded that 15N retention from intravenously administrated
leucine was much higher than from 15N in urea given with feed, as estimated previously.
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