SHORT COMMUNICATION
Isolated digesta as an effective and reliable ex vivo system
to study the release profile of drug formulations:
study on Candida utilis urate oxidase
S. Pierzynowski
1,3,8
1
Lund University, Department of Biology, Sölvegatan 35, 22362, Lund, Sweden
2
The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, Department of Animal Physiology,
Instytucka 3, 05-110, Jabłonna, Poland
3
Anara AB, Alfågelgränden 24, 23132, Trelleborg, Sweden
4
Institute of Clinical Dentistry, Fondazione Policlinico Universitario A. Gemelli IRCCS, Universita Cattolica del Sacro Cuore,
Oral Surgery and Implantology Unit, Division of Oral Surgery and Implantology, Department of Head and Neck,
Largo Francesco Vito, 1, 00168 Rome, Italy
5
Vasyl Stefanyk Precarpathian National University, Department of Biochemistry and Biotechnology,
Shevchenka St 57, 76000, Ivano-Frankivsk, Ukraine
6
Taras Shevchenko National University, Department of Fundamental Medicine,
Akademika Hlushkova Avenue 2, 03022, Kyiv, Ukraine
7
University of the Witwatersrand, School of Physiology, Faculty of Health Sciences,
7 York Road Parktown, 2193, Johannesburg, South Africa
8
Institute Rural Medicine, Department of Biology, Doktora Kazimierza Jaczewskiego 2, 20090, Lublin, Poland
Publication date: 2024-09-13
Corresponding author
KEYWORDS
TOPICS
ABSTRACT
Many artificial systems simulate the physiological state of the
human gastrointestinal tract (GIT) and its separate compartments. These
systems have the biorelevant media and imitate the physical forces and transit
times of the GIT compartments, however, they lack the food-related and withincompartmental
regulations and thus issues with translation of the data obtained
to clinics arise. We aimed to introduce an alternative, simple and reliable ex vivo
system which can be used in a laboratory setting, using fresh chyme from fed
or fasted animals (pigs) to study the release profile of various drugs. For the
present study we used porcine chyme collected from different gut compartments
(stomach, duodenum and ileum) of six cross-bred male pigs in the fed state.
Five different formulations of urate oxidase from Candida utilis were used as
examples of tested drug substances. The performance of each formulation
was tested by incubation in chyme at 37 °C for up to 4 h in the presence of
uric acid. Samples were taken during the whole incubation time and the uric
acid levels were estimated. The proposed ex vivo system provides information
about the stability and performance of active drug substances in different gut
compartments and can be used to test different formulations, assess possible
drug-drug interactions, and the effects of fed and fasted conditions on the test
substance of interest in the small and large intestine, taking into consideration
diet-related changes in GIT secretions and intercompartmental regulation.
CONFLICT OF INTEREST
The Authors declare that there is no conflict of interest.
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