ORIGINAL PAPER
Ligand-binding activity of growth hormone
receptor (GH-R) in bulls of different breeds with
identified GH-R genotypes
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1
Institute of Genetics and Animal Breeding, Polish Academy of Sciences,
Jastrzębiec, 05-551 Mroków, Poland
2
The Kielanowski Institute of Animal Physiology and Nutrition,
Polish Academy of Sciences,
05-110 Jabłonna, Poland
Publication date: 2002-05-09
J. Anim. Feed Sci. 2002;11(2):223-236
KEYWORDS
ABSTRACT
The relationship between the ligand-binding activity of growth hormone receptor (GH-R) and
polymorphism Alul, Accl and Stul sites in the 5' flanking region of bovine GH-R gene was examined. The study was performed on twenty six 15-month old bulls of dairy (Friesians) and beef breeds
(Charolais, Limousine, Piemontese, Aberdeen Angus, Hereford). Receptor binding capacity (Bmax)
and dissociation constant (Kd) for GH-R were determined by the Scatchard method. The polymorphism in the GH-R gene was analyzed by PCR-RFLP technique. Our results showed significant
differences in the liver GH-R Bmax and Kd between the dairy and beef breeds. Bmax was greater (P≤0.05)
in Polish Friesians as compared to the beef breeds, while Kd revealed the lowest figure in the dairy
breed (P≤0.01). No significant differences were found in the Bmax and Kd values between genotypes
within the 5' flanking region of bovine GH-R gene. In conclusion, ligand-binding activity of the liver
GH receptors was shown to differ between the dairy and beef bulls. Furthermore, the polymorphism
in the 5' regions of GH-R gene seems to have no influence on ligand-binding properties of the liver GH
receptor in cattle.
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