SHORT COMMUNICATION
Methane production in in vitro studies as an effect
of different additives to grass-clover silage
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1
The August Cieszkowski Agricultural University of Poznań, Department of Animal Nutrition and Feed Management,
Wołyńska 33, 60-637 Poznań, Poland
2
The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences,
05-110 Jabłonna, Poland
3
The August Cieszkowski Agricultural University of Poznań, Department of Biotechnology and Food Microbiology, Wojska Polskiego 48, 60-237 Poznań, Poland
Publication date: 2005-06-21
Corresponding author
A. Cieślak
The August Cieszkowski Agricultural University of Poznań, Department of Animal Nutrition and Feed Management,
Wołyńska 33, 60-637 Poznań, Poland
J. Anim. Feed Sci. 2005;14(Suppl. 1):235-238
KEYWORDS
ABSTRACT
The effects of biological (VTT) and chemical (KemiSile 2000) additives to grass-clover silage on
methane emission, basic rumen fermentation pattern, rumen bacteria and protozoa were investigated
using a batch culture system. Supplementing grass-clover silage with either additive significantly
increased (P≤0.05) in vitro dry matter disappearance (IVDMD) and bacteria count. Overall, neither
of the additives affected the VFA proportion. The only significant differences (P≤0.05) were in
the isovalerate percentage and acetate:propionate ratio (A/P). Increased A/P and IVDMD levels,
bacteria count and methane emission were found in both groups.
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