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ORIGINAL PAPER
Microbial inoculant and an extract of Trichoderma longibrachiatum
with xylanase activity effect on chemical composition,
fermentative profile and aerobic stability
of guinea grass (Pancium maximum Jacq.) silage
1
Federal University of Grande Dourados, Department of Animal Sciences, Dourados - MS, 79825-070, Brazil
2
Federal University of Grande Dourados, Department of Biological and Environmental Science, Dourados - MS, 79825-070, Brazil
3
Kansas State University, Department of Animal Sciences and Industry, Manhattan, KS 66506-8028, USA
4
University of São Paulo, Departament of Animal Nutrition and Production, 13635-900 Pirassununga, São Paulo, Brazil
Publication date: 2017-12-08
Corresponding author
J. R. Gandra
Federal University of Grande Dourados, Department of Animal Sciences, Dourados - MS, 79825-070, Brazil
Federal University of Grande Dourados, Department of Animal Sciences, Dourados - MS, 79825-070, Brazil
J. Anim. Feed Sci. 2017;26(4):339-347
KEYWORDS
silagePanicum maximumfibrolytic enzymelactic acid bacteriaLactobacillus plantarumPediococcus acidilactici
TOPICS
ABSTRACT
The objective of this study was to determine the effects of increasing
dose of a microbial inoculant alone or in combination with a Trichoderma
longibrachiatum extract with xylanase activity on total losses, chemical
composition, fermentative profile, microbiological quality and aerobic stability
of guinea grass (Panicum maximum Jacq. cv. Mombasa) silage. Sixty minisilos
(0.022 m3) were used in a 3 × 2 factorial experiment, composed by three
levels (0, 4 or 8 g · t−1 of fresh forage) of microbial inoculant (INO) and two
levels (0 or 1 IU · g−1 of fresh forage) of enzyme product (ENZ). INO consisted
of Lactobacillus plantarum at 4 × 1010 cfu · g−1 and Pediococcus acidilactici at
4 × 1010 cfu · g−1. Silos were opened after 60 days. The combination of INO8 with
ENZ caused the lowest gas losses. ENZ increased silage crude protein content,
as well as the dry matter and neutral detergent fibre (NDF) in vitro digestibility.
INO doses exerted a positive quadratic effect on NDF in vitro digestibility.
ENZ addition increased acetic acid concentration, while INO treatments linearly
decreased acetic and butyric acid concentrations and linearly increased lactic
and propionic acid concentrations in silage. INO exhibited a negative quadratic
effect on pH and NH3-N concentration of guinea grass silage and positive linear increase in the counts of anaerobic bacteria. Combinations of ENZ and INO8
decreased silage aerobic stability. Although there was observed no combined
effect of ENZ and INO on silage chemical composition and fermentative profile,
they exerted positive influence on NDF in vitro digestibility of the guinea grass
silage when added alone (ENZ and INO at a dose of 4 g · t−1).
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| ISSN: | 1230-1388 |
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