The experiment was conducted on 16 Red-and-White cows in a 2 x 2 Latin square design, using two levels of CaFA salts (without and with 6% salt in DM) and a low or high level of copper (13 vs 61 mg Cu/kg DM).The cows were fed a grass-maize (1:1) silage and a concentrate given in the amount of 0.28 kg/kg milk yield. DM intake averaged 16.79 ± 0.15 kg, including 10.63 ± 0.12 kg from silage and 6.18 ± 0.14 kg/d^-1 from the concentrate. The average FCM milk yield was 22.84 ± 0.54 kg/d^-1 with 4.11 ± 0.44% fat, 3.01 ± 0.33% protein and 5.36 ± 0.34% lactose contents. A significant (P = 0.028), negative effect of copper on the fat content of milk was found, whereas CaFA salts had a positive effect on the daily synthesis of fat and protein. The total cholesterol content in milk equalled 13.64 ± 0.40 mg/100 g and did not differ significantly at the levels of CaFA or copper used in the diet. Feeding cows CaFA salts lowered the level of saturated fats in milk (capric (CI0), lauric (C12), myristic (CI4) and palmitic (C16) acids), and raised the level of stearic (C18) and alpha-linolenic (C18:3, n-3) acids. The higher level of copper significantly (P = 0.0001) increased the content of oleic acid (C18:1) in milk. Feeding cows CaFA increased the level of unsaturated fatty acids (UFA, MUFA) in milk in a highly significant manner, as did higher doses of copper (P = 0.0001). The level of fatty acids exhibiting hypocholesterolemic properties (DFA) increased from 40.86 to 46.85% under the influence of CaFA and from 43.14 to 44.57% under the influence of copper.