Ten groups of 36 Tetra SL hens were caged individually. They were fed 10 diets containing 17% crude protein and 11.6 MJ ME/kg, in which the cereals were: wheat or maize only, or the following cereal mixes (1:1): wheat and maize; barley and naked oats, or barley; regular or naked oats in combination with wheat or maize. The chemical composition of cereals, the fatty acid profiles in grain cereals, blended fat, and of the yolks of 12 eggs from each group were determined. The content of n-6 and n-3 fatty acids and their ratio in egg-yolk lipids varied widely according to the type of cereal and level of animal fat in the diet. Diets containing barley with naked oats or wheat, and maize with regular oats, supplemented with adequate amounts of blended animal fat are considered favourable in terms of increasing the level of n-3 fatty acids and decreasing the PUFA n-6/n-3 ratio in egg yolks.