Molecular identification, sequence analyses, tissue
expression profile of a novel cattle gene-GBE1 in
Yunnan cattle

The complete coding sequences of a cattle gene - the glucan (1,4-alpha-), branching enzyme 1 (glycogen branching enzyme), GBE1, was amplified using the reverse transcriptase polymerase chain reaction (RT-PCR) based on the conserved coding sequence information of the human, mouse and highly homologous cattle ESTs. Sequence analysis revealed that cattle GBE1 has high homology with the GBE1 from six species: dog (92%), cat (91%), horse (91%), human (89%), mouse (88%) and rat (88%). The phylogenetic tree analysis revealed that the cattle GBE1 has closer genetic relationships with the GBE1 of dog and cat. Tissue expression profile analysis revealed that the cattle GBE1 gene was highly expressed in liver, moderately expressed in muscle and fat, weakly expressed in kidney, small intestine, large intestine, heart and lung. Our experiment established the primary foundation for further research on the cattle GBE1 gene.

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1230-1388

Assigning DOI numbers, introducing articles from supplements and recent publications to POL-index database, maintaining anti-plagiarism detection, electronic system to proceed manuscripts and webpage of the Journal with interactive pdf files, and language correction of manuscripts published in Journal of Animal and Feed Sciences are financed in the years 2018–2019 by the Ministry of Science and Higher Education from the funds for science popularization activities, Agreement No. 631/P-DUN/2018.