The effect of supplementing threonine (Thr) to wheat-based diets containing about 18 (HP) and 16% (LP) protein was determined in pigs with high growth potential (barrows of synthetic line 990). Two basal, Thr deficient HP and LP diets of about 13 MJ ME/kg were used in digestibility experiment for determination of true ileal digestible amino acids content. Based on these results, four HP diets containing 0.86% true ileal digestible lysine and four Thr levels, and two LP diets with two Thr levels were formulated for N balance experiment (Trials 1 and 2, respectively) from the same ingredients and crystalline amino acids. In HP diets true digestible threonine:lysine ratios were 0.53, 0.60, 0.65 and 0.70, and in LP diets were of 0.53 and 0.65. Each trial was performed during 35 days on 6 pigs (per diet) in the range of body weight (BW) from about 25 to 50 kg with N balance at 35 and 45 kg of BW. In pigs fed on HP diets no response was found to increasing Thr, either in N retention or in pig performance. In contrast, in pigs fed the LP diets, Thr supplementation increased daily N retention from 19.2 to 20.7 g (P≤0.03) at 35 kg of BW. The effect of Thr supplementation on pig performance was not significant, however, it tended to increase average daily gain (774 vs 798 g) and lower feed/gain ratio (2.03 vs 1.97). The pigs fed LP diet excreted about 22% N less than the pigs fed on HP diet. In conclusion, growing pigs with high lean gain potential can optimize their nitrogen retention and growth performance on diets with protein level decreased to 16% and supplemented to contain about 0.86 and 0.56% of true ileal digestible lysine and threonine, respectively.