ORIGINAL PAPER
Quantitative analysis of oestrogen receptor
α and β gene expression during prolonged culture
of pig granulosa cells
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1
Institute of Animal Science,
Department of Biology of Reproduction, Přátelství 815, 104 00 Prague-Uhříněves, Czech Republic
2
Institute of Animal Science, Department of Molecular Genetics
Přátelství 815, 104 00 Prague-Uhříněves, Czech Republic
Publication date: 2012-06-18
Corresponding author
E. Chronowska
Institute of Animal Science,
Department of Biology of Reproduction, Přátelství 815, 104 00 Prague-Uhříněves, Czech Republic
J. Anim. Feed Sci. 2012;21(2):313-323
KEYWORDS
ABSTRACT
The aim of the present work was to determine the relative transcript abundance of the oestrogen
receptor alpha (ERα) and beta (ERβ) gene in long-term cultures (up to 18 days) of pig granulosa
cells (GC) obtained from small (1-2 mm; SF-GC) and large (5-7 mm; LF-GC) follicles. GC were
cultured in the presence of 10% foetal calf serum (FCS) and one of the following growth factors:
leukaemia inhibitory factor (LIF), basic fibroblast growth factor (bFGF), or stem cell factor (SCF).
The relative transcript abundance of the investigated genes was measured by real time PCR (RTPCR).
In comparison with the control (medium only), LIF significantly increased (P<0.05-0.01)
ERα gene expression in SF-GC after 3, 15 and 18 days of culture and after 12 days in LF-GC. The
ERβ mRNA level in SF-GC was significantly decreased (P<0.01) by all of the investigated factors
after 3, 6, 9 and 12 days of incubation. In conclusion, expression of both types of oestrogen receptors
during prolonged culture of pig GC under different conditions suggests the role of oestrogens acting
via their receptors in maintaining survivability of porcine granulosa cells in vitro.
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