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ORIGINAL PAPER
Quantitative assessment of Yersinia enterocolitica
in raw pork meat using real time PCR (qPCR) technique
1
Lomza State University of Applied Sciences, Akademicka 14, 18-400 Łomża
Publication date: 2017-06-14
Corresponding author
J. Anim. Feed Sci. 2017;26(2):141-147
KEYWORDS
TOPICS
ABSTRACT
The aim of the present study was to develop a highly sensitive and
specific TaqMan probe with a set of primers for detection of pathogenic Yersinia
enterocolitica strains and to eliminate the pre-PCR enrichment step in the realtime
PCR (qPCR). A newly developed qPCR assay which is sensitive and specific
for quick and reliable quantitative assessment of Y. enterocolitica present
in artificially contaminated raw pork meat samples is described. This protocol
involves the qPCR method with a TaqMan probe. The primers and probe were
designed on the base of locus_tag CH49_3099 gene. This protocol appeared
to be reliable for both intended applications: 1. identification and quantification
of Y. enterocolitica in artificially and naturally contaminated raw pork meat and
2. establishment of growth potentials of different serotypes of Y. enterocolitica
in raw meat at the usually used storage temperatures. This developed method
makes it possible to eliminate the pre-PCR enrichment step enabling for the
rapid assessment of meat-related consumer exposure to this pathogen.
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CITATIONS (1):
1.
Isolation, characterization and antimicrobial resistance of Yersinia enterocolitica from Polish cattle and their carcasses
Piotr Łada, Klaudia Kończyk-Kmiecik, Agata Bancerz-Kisiel
BMC Veterinary Research
Piotr Łada, Klaudia Kończyk-Kmiecik, Agata Bancerz-Kisiel
BMC Veterinary Research
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