The reverse transcription polymerase chain reaction (RT-PCR) is one of the most sensitive
methods for the detection and quantitation of mRNA. It is widely used for quantification of mRNA
levels and is useful tool for basic research, agriculture, medicine and biotechnology. Introduction of
Real-time technique significantly improves rapidity, sensitivity, specificity and reproducibility of RT-PCR method. Real-time PCR detects and quantifies nucleic acids even from live and dead pathogens
or cells. This review discusses the background, advantages and limitations of conventional and Real-time RT-PCR methods for quantitation of gene expression and its application in basic, agricultural
and biomedical research.
CITATIONS(1):
1.
Development of an Assay to Quantify Rumen Ciliate Protozoal Biomass in Cows Using Real-Time PCR John T. Sylvester, Sanjay K. R. Karnati, Zhongtang Yu, Mark Morrison, Jeffrey L. Firkins The Journal of Nutrition
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