The aim of the study was to evaluate the influence of feeding different protein levels (11.4, 13.3 and 16.9%) in diets for goats on the fatty acid (FA) profile in milk. The experimental design was 3 × 3 Latin square. Each 22-day experimental period consisted of 14 days adaptation to the diets and 8 days for milk sample collection. Separation of methylated FAs was achieved using GLC. Milk production was nearly the same when the low- and medium-protein diets were fed, while the highprotein diet resulted in a tendency to increase milk production. The concentrations of total saturated fatty acids (SFA) were similar in milk from goats fed the diets containing low- and medium-protein contents, while the diet containing the highest content of protein resulted in a slight increase in the concentration and daily production of SFA milk. The concentration and daily production of monounsaturated fatty acids, CLA isomers, other polyunsaturated fatty acids and the sum of FAs in milk increased as the dietary protein level rose. The highest dietary protein content resulted in an increase in the Δ9-desaturase index.