Rumen degradability of protein and amino acids were estimated using the nylon bag technique in four different roughages: cocksfoot, vetch whole crop, clover grass, and lucerne pellets after incubation in the rumen for 4 or 16 h, respectively. Intestinal digestibility of protein and amino acids were estimated by the mobile nylon bag technique in the intact protein and in residues pre-incubated in the rumen for 16 h. Degradability of total amino acid nitrogen followed the same trend as for nitrogen, whereas notable differences were observed for individual amino acids, especially for methionine. Intestinal digestibility of intact feed protein varied between 0.820 and 0.887 for nitrogen, and between 0.869 and 0.931 for total amino acid nitrogen. Among the individual amino acids only cystine differed markedly from the mean value for total amino acids. Intestinal digestibility of 16 h rumen pre-incubated protein was much lower than for the intact protein and varied between 0.444 and 0.748 for nitrogen, and between 0.559 and 0.844 for total amino acid nitrogen. In all samples tested a higher digestibility was observed for total amino acid nitrogen compared to total nitrogen.