SHORT COMMUNICATION
Shifts of rumen microbial population detected by
real-time PCR when methanogenesis is inhibited
1
Institute of Dairy Science, Ministry of Education Key Laboratory of Molecular Animal Nutrition,
Zhejiang University, Hangzhou 310029, P.R. China
2
Gastrointestinal Microbiology Laboratory, Nanjing Agricultural University,
Nanjing 210095, P.R. China
3
CSIRO Livestock Industries,
306 Carmody Rd. St Lucia, QLD, 0467, Australia
Publication date: 2007-09-17
Corresponding author
J. X. Liu
Institute of Dairy Science, Ministry of Education Key Laboratory of Molecular Animal Nutrition, Zhejiang University, Hangzhou 310029, P.R. China
Institute of Dairy Science, Ministry of Education Key Laboratory of Molecular Animal Nutrition, Zhejiang University, Hangzhou 310029, P.R. China
J. Anim. Feed Sci. 2007;16(Suppl. 2):107-112
KEYWORDS
ABSTRACT
Real-time PCR was conducted to quantify methanogens, bacteria, Fibrobacter succinogenes,
Ruminococcus flavefaciens and fungi in rumen fluid incubated in vitro when methane synthesis
was inhibited. After 24 h incubation, methanogens in sodium salt of 2-bromoethanesulphonic acid
(BES)-added fluids was reduced to 10.4% of control (P<0.01). BES did not significantly influence
the number of bacteria and R. flavefaciens. However, the number of F. succinogenes in BES-treated
fluid was 50.0% higher than in control (P<0.01). Population density of fungi fell by 61.9% (P<0.01)
in comparison with control. Addition with BES changed the rumen microbiota and had a varying
influence on different microbes.
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