Suitable method for simultaneous and specific
detection of maize ( Zea mays  l.) and genetically
modified soyabean ( Glycine max  l.) in animal feeds

Present method allows a simple and rapid identification of maize and specific transgenic soyabean event in raw materials and processed feed samples. The method combines two aspects: suitable DNA purification and amplification by means of multiplex RT-PCR. Efficiency and accuracy of this method have been tested and the limit of detection (LOD) reached was of 0.1 ng/μl for one of the species and of 1 ng/μl for the other one. The checking of the method has been realized by means of RT-PCR using the specific probes for each one of the systems by means of comparison with certified reference materials. The obtained results showed that with this method it is possible to obtain, in a shorter time, quality values for both: identification and quantification of GMOs, in raw and processed samples.

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Universal DNA-Based Methods for Assessing the Diet of Grazing Livestock and Wildlife from Feces
Anthony Pegard, Christian Miquel, Alice Valentini, Eric Coissac, Frédéric Bouvier, Dominique François, Pierre Taberlet, Erwan Engel, François Pompanon
Journal of Agricultural and Food Chemistry

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1230-1388

Assigning DOI numbers, introducing articles from supplements and recent publications to POL-index database, maintaining anti-plagiarism detection, electronic system to proceed manuscripts and webpage of the Journal with interactive pdf files, and language correction of manuscripts published in Journal of Animal and Feed Sciences are financed in the years 2018–2019 by the Ministry of Science and Higher Education from the funds for science popularization activities, Agreement No. 631/P-DUN/2018.