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2/2016 vol. 25
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ORIGINAL PAPER
The apelin-13 influences the activity of pancreatic enzymes in young rats
H. Antushevich 1
,
 
M. Kapica 2
,
 
A. Kuwahara 3
,
 
I. Kato 3
,
 
A. Krawczyńska 1
,
 
A. P. Herman 1
,
 
B. Pawlina 1
,
 
R. Zabielski 4
 
 
 
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1
The Kielanowski Institute of Animal Physiology and Nutrition Polish Academy of Sciences, Instytucka 3, 05-110 Jabłonna, Poland
 
2
University of Life Sciences, Faculty of Veterinary Medicine, Department of Animal Physiology, Akademicka 12, 20-950 Lublin, Poland
 
3
University of Shizuoka, Laboratory of Physiology, 422-8526 Shizuoka, Japan
 
4
Warsaw University of Life Sciences - SGGW, Faculty of Veterinary Medicine, Department of Physiological Sciences, Nowoursynowska 166, 02-766 Warsaw, Poland
 
 
Publication date: 2016-05-22
 
 
Corresponding author
H. Antushevich   

The Kielanowski Institute of Animal Physiology and Nutrition Polish Academy of Sciences, Instytucka 3, 05-110 Jabłonna, Poland
 
 
J. Anim. Feed Sci. 2016;25(2):160-166
DOI: https://doi.org/10.22358/jafs/65576/2016
 
 
Article (PDF)
References (38) Citations (3)
 
KEYWORDS
Apelin
CCK
lipase
pancreas
stomach
young rats
 
ABSTRACT
Apelin and its APJ receptor are present inter alia in colostrum and in the young animal gastrointestinal tract. This peptide exerts numerous effects participating in the appetite and drinking behaviour, gastric acid and insulin secretion. The aim of the study was to investigate the effect of apelin-13 on the activity of pancreatic and gastric enzymes in young animals. The two experiments were carried out on weaning Wistar rats (50 ± 10 g) which received the apelin-13 or physiological saline (in the corresponding control groups) by intragastric or intraperitoneal way twice a day for 10 days (100 nmol · kg–1 body weight). At the end of each experiment rats were sacrificed and blood samples were collected for apelin and cholecystokinin (CCK) radioimmunoassay. The fragments of the pancreas and stomach were weighted and frozen for the further digestive enzymes activity analysis. The intragastric and intraperitoneal administration of apelin-13 increased plasma CCK level in young rats. The intraperitoneal injection of apelin-13 stimulated pancreatic trypsin, α-amylase and lipase activity, but had no effect on the activity of gastric enzymes. On the other hand, the intragastric administration of apelin-13 stimulated only the activity of pancreatic lipase and had no effect on the activity of gastric pepsin and rennet. So, circulating apelin exerts the most pronounced effect on pancreatic enzymes activity in young rats, but neither circulating nor luminal apelin influences gastric enzymes activity. Regardless the route of administration, apelin stimulates lipase activity which points out its considerable role in the regulation of the fat digestion.
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CITATIONS (3):
1.
Peripheral apelin-13 administration inhibits gastrointestinal motor functions in rats: The role of cholecystokinin through CCK 1 receptor-mediated pathway
Mehmet Bülbül, Osman Sinen, İlknur Birsen, V. Nimet İzgüt-Uysal
Neuropeptides
 
CrossRef
 
 
2.
Exogenous obestatin affects pancreatic enzyme secretion in rat through two opposite mechanisms, direct inhibition and vagally-mediated stimulation
M. Kapica, I. Puzio, I. Kato, A. Kuwahara, R. Zabielski, H. Antushevich
Journal of Animal and Feed Sciences
 
CrossRef
 
 
3.
Review: Apelin in disease
Hanna Antushevich, Maciej Wójcik
Clinica Chimica Acta
 
CrossRef
 
 
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